Strang G, Rickinson A B
Department of Cancer Studies, University of Birmingham, U.K.
Immunology. 1987 Dec;62(4):647-54.
The cytotoxic T-cell response induced by primary Epstein-Barr virus (EBV) infection and detectable in the blood of infectious mononucleosis (IM) patients shows several unusual features when tested in in vitro assays. Lysis of EBV-transformed target lines occurs with no apparent HLA restriction, and the putative EBV specificity of the response has been seriously questioned. In the present work we show that the primary T-cell response in IM is polyclonal and indeed does contain a virus-specific HLA class I antigen-restricted component, which can be selectively expanded in vitro in the presence of appropriate stimulator cells and IL-2. This allows functional analysis of the virus-specific component of the response in the absence of co-resident reactivities. Studies on blood samples taken from individuals in the acute phase of IM and again post-convalescence suggest that functionally similar populations of HLA class I-restricted cytotoxic T cells are involved in the control of both the primary and persistent phase of EBV infection.
原发性爱泼斯坦-巴尔病毒（EBV）感染所诱导并可在传染性单核细胞增多症（IM）患者血液中检测到的细胞毒性T细胞反应，在体外试验中检测时呈现出几个不同寻常的特征。EBV转化的靶细胞系的裂解发生时没有明显的HLA限制，并且该反应假定的EBV特异性受到了严重质疑。在本研究中，我们表明IM中的原发性T细胞反应是多克隆的，并且确实包含一种病毒特异性的HLA I类抗原限制成分，在存在适当的刺激细胞和白细胞介素-2的情况下，该成分可在体外被选择性扩增。这使得在不存在共存反应性的情况下对反应的病毒特异性成分进行功能分析成为可能。对急性期IM患者以及恢复期后再次采集的血样进行的研究表明，功能相似的HLA I类限制细胞毒性T细胞群体参与了EBV感染的原发性和持续性阶段的控制。
