De Boisblanc B P, Dobrescu C, Skrepnik N, Nelson S, Spitzer J J, Bagby G J
Department of Medicine, Louisana State University Medical Center, New Orleans 70112, USA.
Am J Physiol. 1996 Mar;270(3 Pt 1):L452-8. doi: 10.1152/ajplung.1996.270.3.L452.
The rate of glucose utilization (Rg) of various tissues including lung and bronchoalveolar lavage (BAL) fluids cells was measured, using the 2-deoxyglucose technique in Sprague-Dawley rats 4 h after challenge with either 1 mg/kg intravenous or 0.3 mg/kg intratracheal lipopolysaccharide (LPS). After intravenous LPS, Rg increased in whole lung and nonrespiratory tissues, but was unaltered in BAL cells. After intratracheal LPS, the Rg of nonrespiratory tissues was unchanged, but the Rg of BAL cells increased from 3.7 +/- 0.3 to 71.5 +/- 16.0 nmol/min. This increase in the Rg of BAL cells was explained by a doubling of the macrophage specific Rg, by a 100-fold increase in polymorphonuclear leukocytes (PMN) number, and by a higher Rg in PMN than in macrophages. These results demonstrate that increased glucose utilization after intratracheal LPS is confined to the respiratory system and that intra-alveolar phagocytes participate in this increase.
采用2-脱氧葡萄糖技术，在给予1mg/kg静脉注射或0.3mg/kg气管内注射脂多糖（LPS）4小时后的Sprague-Dawley大鼠中，测量包括肺和支气管肺泡灌洗（BAL）液细胞在内的各种组织的葡萄糖利用率（Rg）。静脉注射LPS后，全肺和非呼吸组织中的Rg增加，但BAL细胞中的Rg未改变。气管内注射LPS后，非呼吸组织的Rg未改变，但BAL细胞的Rg从3.7±0.3增加到71.5±16.0nmol/分钟。BAL细胞Rg的这种增加是由于巨噬细胞特异性Rg加倍、多形核白细胞（PMN）数量增加100倍以及PMN中的Rg高于巨噬细胞。这些结果表明，气管内注射LPS后葡萄糖利用率的增加仅限于呼吸系统，并且肺泡内吞噬细胞参与了这种增加。
