Gas chromatography-mass spectrometry of metabolites in hemodialysis fluid.

An analytical method has been developed for the separation and identification of several metabolites in used hemodialysis fluid obtained during the treatment of a uremic patient on the artificial kidney. The procedure involves ion exchange, evaporation, and trimethylsilylation; the dervatized components were studied by combined gas chromatography--mass spectrometry. Twelve compounds were satisfactorily resolved; six were conclusively identified from mass spectral data. The identified components include phosphoric acid, glucopyranurono-(6 leads to 1)-lactone, citric acid, D-gluconic acid-beta-lactone, alpha-D-glucose, and beta-D-glucose. A seventh component was tentatively identified as mannonic acid.