评估胸苷激酶（tk）基因座作为高度减毒痘苗病毒MVA株插入位点的情况。
Evaluation of the thymidine kinase (tk) locus as an insertion site in the highly attenuated vaccinia MVA strain.
作者信息
Scheiflinger F, Falkner F G, Dorner F
机构信息
Biomedical Research Center, Immuno AG, Orth/Donau, Austria.
出版信息
Arch Virol. 1996;141(3-4):663-9. doi: 10.1007/BF01718324.
PMID:8645102
Abstract
The highly attenuated 'modified vaccinia Ankara' (MVA) strain is a potential live vaccine vector. Insertional inactivation of the tk-gene resulted in viruses difficult to purify. Co-integration of a functional fowlpox virus tk-gene allowed easy generation of recombinants, indicating that the genetically stable tk-gene region is a suitable insertion site, if tk-gene activity is substituted.
摘要
高度减毒的“安卡拉痘苗病毒”（MVA）株是一种潜在的活疫苗载体。tk基因的插入失活导致病毒难以纯化。功能性禽痘病毒tk基因的共整合使得重组体易于产生，这表明如果tk基因活性被替代，基因稳定的tk基因区域是一个合适的插入位点。
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