Balter N J, Schwartz S L
J Pharmacol Exp Ther. 1977 Jun;201(3):636-43.
Incubation of mouse peritoneal macrophages with l-[3H]norepinephrine resulted in an apparent multiphasic accumulation of the catecholamine by the cells. Low temperature did and iodoacetate did not inhibit uptake. Part of the accumulation followed saturation kinetics; cocaine, metanephrine and phenoxybenzamine did not inhibit the observed uptake. On this basis, it was concluded that the uptake process or combination of processes could not be classified solely as uptake1, uptake2 or simple diffusion However, similarities between norepinephrine accumulation by macrophages and that by other extraneuronal tissues do exist. This, along with previous morphological data, suggests that the macrophage uptake may typify a component of extraneuronal accumulation of norepinephrine and that this component may, in part, result from a nonspecific membrane perturbation by norepinephrine.
用l-[3H]去甲肾上腺素孵育小鼠腹腔巨噬细胞，会导致细胞对儿茶酚胺出现明显的多相性蓄积。低温可抑制摄取，而碘乙酸则不能。部分蓄积遵循饱和动力学；可卡因、间甲肾上腺素和酚苄明不能抑制所观察到的摄取。基于此，得出的结论是，摄取过程或多个过程的组合不能单纯归类为摄取1、摄取2或简单扩散。然而，巨噬细胞对去甲肾上腺素的蓄积与其他非神经组织对去甲肾上腺素的蓄积之间确实存在相似之处。这一点，连同先前的形态学数据表明，巨噬细胞摄取可能代表去甲肾上腺素非神经蓄积的一个组成部分，并且该组成部分可能部分源于去甲肾上腺素对膜的非特异性扰动。
