Pullen R G, Schofield M, Markham A, Lough J, Menton K
Section of Neuroscience, University of Sunderland, Tyne & Wear, England.
Life Sci. 1996;58(23):2125-35. doi: 10.1016/0024-3205(96)00206-8.
These experiments have investigated selenium movement between blood and CNS in anaesthetised rats. Each animal was surgically anaesthetised and the left femoral blood vessels cannulated for blood withdrawal and solute infusion. Each rat received 75-selenium as sodium selenite infused in normal saline and experiments lasted between 5 minutes and 5 hours during which blood samples were periodically taken. At termination, the CNS was removed, regionally dissected and analysed with the plasma samples for 75-Se radioactivity by gamma-counting. Data were analysed by graphical analysis. Results showed unidirectional uptake of 75-Se into the CNS and regional differences were not found except for the hypothalamus. On average the CNS influx rate constant (Kin) was about 7 +/- 1 x 10(-5) ml/min/g. Data suggest that the 75-Se most likely entered the CNS as a free ionic form.
这些实验研究了麻醉大鼠血液与中枢神经系统（CNS）之间硒的转运情况。每只动物均通过手术进行麻醉，并将左股血管插管用于采血和溶质注入。每只大鼠接受以亚硒酸钠形式注入生理盐水的75-硒，实验持续5分钟至5小时，在此期间定期采集血样。实验结束时，取出中枢神经系统，进行区域解剖，并与血浆样本一起通过伽马计数分析75-硒的放射性。数据通过图形分析进行处理。结果显示75-硒单向摄取进入中枢神经系统，除下丘脑外未发现区域差异。平均而言，中枢神经系统流入速率常数（Kin）约为7±1×10⁻⁵毫升/分钟/克。数据表明75-硒最有可能以游离离子形式进入中枢神经系统。
