Pullen R G, Schofield M, Markham A, Lough J, Menton K
Section of Neuroscience, University of Sunderland, Tyne & Wear, England.
Neurochem Res. 1995 Oct;20(10):1141-6. doi: 10.1007/BF00995376.
These experiments have investigated selenium movement between blood and the CNS in anaesthetized rats. Each animal was anaesthetized and the left femoral blood vessels cannulated for blood withdrawal and solute infusion. Each rat received 75-Se as sodium selenite infused in normal saline and experiments lasted between 5 minutes and 5 hours during which blood samples were periodically taken. At termination, the CNS was removed, dissected and analysed with the plasma samples for 75-Se radioactivity by gamma-counting. Data were analyzed by multiple-time uptake analysis. Results showed unidirectional uptake of 75-Se into the CNS and some regional differences were found. On average the CNS influx rate constant (Kin) was about 7 +/- 1 x 10(-5) ml/min/g. This indicates that the 75-Se most likely entered the CNS in a protein-bound form.
这些实验研究了麻醉大鼠血液与中枢神经系统（CNS）之间硒的转运情况。每只动物均被麻醉，左股血管插管用于采血和溶质注入。每只大鼠接受以亚硒酸钠形式注入生理盐水中的75 -硒，实验持续5分钟至5小时，在此期间定期采集血样。实验结束时，取出中枢神经系统，进行解剖，并与血浆样本一起通过γ计数分析75 -硒的放射性。数据通过多次摄取分析进行分析。结果显示75 -硒单向摄取进入中枢神经系统，并且发现了一些区域差异。平均而言，中枢神经系统流入速率常数（Kin）约为7±1×10⁻⁵毫升/分钟/克。这表明75 -硒最有可能以蛋白质结合形式进入中枢神经系统。
