Bhatnagar Y M, Mishra P V, Martinez J A, Saxena K M, Wertelecki W
Department of Structural & Cellular Biology, College of Medicine, University of South Alabama, Mobile 36688, USA.
Biochem Mol Biol Int. 1995 Sep;37(1):57-64.
The telomeres of gorilla, chimpanzee and human peripheral blood cells have been examined by hybridization to an oligonucleotide probe, (TTAGGG)4, following conventional and pulsed-field electrophoresis procedures. The MspI site present near the chromosome terminus undergoes methylation in gorilla, chimpanzee and human genome as shown by the HpaII digestion. Minor (TTAGGG)4-hybridizing sequences have been also detected in the chimpanzee HindIII and MspI digests.
采用常规电泳和脉冲场电泳方法，通过与寡核苷酸探针（TTAGGG）4杂交，对大猩猩、黑猩猩和人类外周血细胞的端粒进行了检测。如经HpaII酶切所示，在大猩猩、黑猩猩和人类基因组中，靠近染色体末端的MspI位点会发生甲基化。在黑猩猩的HindIII和MspI酶切产物中也检测到了少量（TTAGGG）4杂交序列。
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