Rearden A, Phan H, Fukuda M
Department of Pathology, University of California, San Diego, La Jolla 92093.
Biochem Genet. 1990 Apr;28(3-4):223-31. doi: 10.1007/BF00561339.
Twelve restriction fragment length polymorphisms (RFLPs) were detected in common chimpanzee using two restriction enzymes (HindIII and MspI) and four DNA probes to the coding regions of the human glycophorin A (GPA) and glycophorin B (GPB) genes and their 3'-untranslated regions. Seven RFLPs correlated with red cell expression of the Vc determinant of the MN blood group-related V-A-B-D system and five RFLPs correlated with nonexpression of this antigen. Animals heterozygous for the V allele that encodes the Vc determinant had all 12 polymorphic restriction fragments and appeared to show reduced intensity of probe hybridization to these fragments, consistent with the presence of a V and a non-V allele. No RFLPs were detected with EcoRI, SstI, or BamHI, in spite of the relatively large segment of DNA (at least 20 kb) involved in the polymorphisms. The RFLPs were chimpanzee specific and were not found in man, gorilla, orangutan, or gibbon. Multiple RFLPs distinguishing primate species are rare and may be useful markers for molecular evolution.
使用两种限制性内切酶（HindIII和MspI）以及针对人类血型糖蛋白A（GPA）和血型糖蛋白B（GPB）基因编码区及其3'非翻译区的四种DNA探针，在普通黑猩猩中检测到了12种限制性片段长度多态性（RFLP）。七种RFLP与MN血型相关的V - A - B - D系统中Vc决定簇的红细胞表达相关，五种RFLP与该抗原的不表达相关。编码Vc决定簇的V等位基因杂合的动物具有所有12种多态性限制性片段，并且似乎显示出探针与这些片段杂交的强度降低，这与存在一个V等位基因和一个非V等位基因一致。尽管多态性涉及相对较大的DNA片段（至少20 kb），但用EcoRI、SstI或BamHI未检测到RFLP。这些RFLP是黑猩猩特有的，在人类、大猩猩、猩猩或长臂猿中未发现。区分灵长类物种所需的多个RFLP很少见，可能是分子进化的有用标记。
