Felländer G, Linde B, Bolinder J
Department of Surgery, Huddinge Hospital, Stockholm, Sweden.
Int J Obes Relat Metab Disord. 1996 Mar;20(3):220-6.
To investigate the feasibility of the microdialysis ethanol perfusion technique for monitoring nutritive blood flow in subcutaneous adipose tissue.
Microdialysis probes were inserted percutaneously into the subcutaneous adipose tissue in 15 non-obese women, and were perfused with 50 mmol/l of ethanol. The experiments were carried out during basal conditions and in conjunction with local vasodilation induced by external heating. The ethanol exchange ratio (ethanol concentration in the outgoing tissue dialysate vs ethanol concentration in the ingoing perfusate) was determined. A comparison was made with the 133Xe clearance technique to assess the adipose tissue blood flow.
At rest, the ethanol exchange ratio in the individual subjects was inversely correlated to the adipose tissue blood flow, as measured with 133Xe wash-out (r = -0.78-0.82, p < 0.05-0.01). When the subcutaneous temperature was increased in a stepwise fashion by external heating, adipose tissue blood flow, as determined with 133Xe clearance, was increased by about 50% and 100%, respectively, above resting values (F = 26.7, p < 0.0001). At the same time, the ethanol exchange ratio was progressively and significantly (F = 24.6, p < 0.0001) reduced. In the individual subjects there was a close negative correlation (r = -0.90-0.94) between the ethanol exchange ratios and the corresponding adipose tissue blood flow values, as measured by 133Xe clearance, in response to local vasodilation.
The microdialysis ethanol perfusion technique provides a valid indicator of small changes within the physiological range in adipose tissue blood flow.
探讨微透析乙醇灌注技术监测皮下脂肪组织营养性血流的可行性。
将微透析探针经皮插入15名非肥胖女性的皮下脂肪组织，并用50 mmol/l乙醇进行灌注。实验在基础条件下进行，并与外部加热诱导的局部血管舒张相结合。测定乙醇交换率（流出组织透析液中的乙醇浓度与流入灌注液中的乙醇浓度之比）。与133Xe清除技术进行比较以评估脂肪组织血流。
静息时，个体受试者的乙醇交换率与用133Xe洗脱法测量的脂肪组织血流呈负相关（r = -0.78 - 0.82，p < 0.05 - 0.01）。当通过外部加热使皮下温度逐步升高时，用133Xe清除法测定的脂肪组织血流分别比静息值增加约50%和100%（F = 26.7，p < 0.0001）。同时，乙醇交换率逐渐且显著降低（F = 24.6，p < 0.0001）。在个体受试者中，乙醇交换率与相应的用133Xe清除法测量的脂肪组织血流值之间存在密切的负相关（r = -0.90 - 0.94），以响应局部血管舒张。
微透析乙醇灌注技术为脂肪组织血流在生理范围内的微小变化提供了一个有效的指标。
