Lancaster F E, Lawrence J F
Food Research Division, Bureau of Chemical Safety, Food Directorate, Health Protection Branch, Health Canada, Ottawa, Ont., Canada.
J Chromatogr A. 1996 May 3;732(2):394-8. doi: 10.1016/0021-9673(95)01332-6.
During a study of natural food colours, a simple and reliable high-performance liquid chromatography system was developed for use with cochineal and annato. An isocratic mobile phase, consisting of methanol and 6% aqueous acetic acid, resolved bixin and norbixin, while a gradient system was used to separate carminic acid and the annato compounds. The carminic acid contents of cochineal extract, carmine and carmine hydrosoluble were determined using an isocratic mobile phase (40:60, v/v). The detection limit for carminic acid in the various products was approximately 100 ng/g. Carminic acid was determined quantitatively in fruit beverages, yogurt and candies. It was demonstrated that, because of decomposition, carminic acid was not suitable for use in candies when manufacturing temperatures above 100 degrees C were required. Most membrane filters are not suitable for use with cochineal solutions, but a cellulose membrane filter did not adsorb carminic acid and was used successfully to remove impurities from water-based cochineal products and food extracts containing carminic acid.
在一项对天然食用色素的研究中，开发了一种简单可靠的高效液相色谱系统，用于胭脂虫红和胭脂树提取物。一种由甲醇和6%乙酸水溶液组成的等度流动相可分离出胭脂树素和降胭脂树素，而采用梯度系统来分离胭脂红酸和胭脂树提取物中的化合物。使用等度流动相（40:60，v/v）测定了胭脂虫提取物、胭脂红和水溶性胭脂红中的胭脂红酸含量。各种产品中胭脂红酸的检测限约为100 ng/g。对果汁饮料、酸奶和糖果中的胭脂红酸进行了定量测定。结果表明，由于分解，当制造温度需要高于100℃时，胭脂红酸不适用于糖果。大多数膜过滤器不适用于胭脂虫溶液，但纤维素膜过滤器不会吸附胭脂红酸，并成功用于从水性胭脂虫产品和含胭脂红酸的食品提取物中去除杂质。
