Shannon E J, Sandoval F
Immunology Research Department, G.W. Long Hansen's Disease Center, Baton Rouge, LA 70894, USA.
Immunopharmacology. 1995 Nov;31(1):109-16. doi: 10.1016/0162-3109(95)00039-7.
Thalidomide significantly increases the quantity of extracellular IL-2 in cultures of human mononuclear cells stimulated with mitogens or antigen. Cells from 7 donors exposed for 2 h to 4.0 micrograms/ml of thalidomide and stimulated for 16-18 h with 20 micrograms/ml of Concanavalin-A (Con-A) averaged producing 187 +/- 49% more IL-2 than cells stimulated with Con-A alone. In similar experimental procedures and comparisons the pg/ml of IL-2 secreted by thalidomide-treated cells from five donors stimulated with 50 ng/ml of Staphylococcal enterotoxin A (SEA) increased by 159 +/- 32%, and the pg/ml of IL-2 secreted by thalidomide-treated cells from 2 donors stimulated with 5.0 micrograms/ml of purified protein derivative of Mycobacterium tuberculosis increased by 120 +/- 4%. Thalidomide also significantly increases the quantity of intracellular IL-2 in cells stimulated with mitogens. Cells exposed to thalidomide and stimulated with Con-A had an increase in intracellular IL-2 of 130% after 8 h and 157% after 12 h in culture; cells stimulated with SEA had an increase in intracellular IL-2 of 120% after 8 h and 182% after 12 h in culture. Thalidomide did not alter the percent of lymphocytes expressing the alpha-chain of IL-2 receptor, nor did it significantly increase incorporation of [3H]thymidine by cells.
沙利度胺显著增加了用丝裂原或抗原刺激的人单核细胞培养物中细胞外白细胞介素-2（IL-2）的量。来自7名供体的细胞暴露于4.0微克/毫升的沙利度胺2小时，并用20微克/毫升的刀豆球蛋白A（Con-A）刺激16 - 18小时，与仅用Con-A刺激的细胞相比，平均产生的IL-2多187±49%。在类似的实验程序和比较中，用50纳克/毫升葡萄球菌肠毒素A（SEA）刺激的5名供体经沙利度胺处理的细胞分泌的IL-2的皮克/毫升增加了159±32%，用5.0微克/毫升结核分枝杆菌纯化蛋白衍生物刺激的2名供体经沙利度胺处理的细胞分泌的IL-2的皮克/毫升增加了120±4%。沙利度胺还显著增加了用丝裂原刺激的细胞内IL-2的量。暴露于沙利度胺并用Con-A刺激的细胞在培养8小时后细胞内IL-2增加了130%，培养12小时后增加了157%；用SEA刺激的细胞在培养8小时后细胞内IL-2增加了120%，培养12小时后增加了182%。沙利度胺没有改变表达IL-2受体α链的淋巴细胞百分比，也没有显著增加细胞对[3H]胸腺嘧啶核苷的掺入。
