构巢曲霉的tamA基因包含一个假定的锌簇基序，该基序对基因功能并非必需。
The tamA gene of Aspergillus nidulans contains a putative zinc cluster motif which is not required for gene function.
作者信息
Davis M A, Small A J, Kourambas S, Hynes M J
机构信息
Department of Genetics, The University of Melbourne, Parkville, Australia.
出版信息
J Bacteriol. 1996 Jun;178(11):3406-9. doi: 10.1128/jb.178.11.3406-3409.1996.
Abstract
Expression of many nitrogen catabolic enzymes is controlled by nitrogen metabolite repression in Aspergillus nidulans. Although the phenotypes of tamA mutants have implicated this gene in nitrogen regulation, its function is unknown. We have cloned the tamA gene by complementation of a new tamA allele. The tamA sequence shares significant homology with the UGA35/DAL81/DURL gene of Saccharomyces cerevisiae. In vitro mutagenesis of sequences encoding a putative zinc cluster DNA binding domain indicated that this motif is not required for in vivo TamA function.
摘要
在构巢曲霉中，许多氮分解代谢酶的表达受氮代谢物阻遏的控制。虽然tamA突变体的表型表明该基因参与氮调节，但其功能尚不清楚。我们通过对一个新的tamA等位基因进行互补克隆了tamA基因。tamA序列与酿酒酵母的UGA35/DAL81/DURL基因具有显著的同源性。对编码假定锌簇DNA结合结构域的序列进行体外诱变表明，该基序对于TamA在体内的功能不是必需的。
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