Polley S D, Caddick M X
Department of Genetics and Microbiology, University of Liverpool, UK.
FEBS Lett. 1996 Jun 17;388(2-3):200-5. doi: 10.1016/0014-5793(96)00541-8.
Mutations within the meaB gene elicit the inappropriate expression of several activities subject to nitrogen metabolite repression in Aspergillus nidulans and also have some unrelated phenotypic effects. We have cloned meaB and isolated a full length cDNA clone. Northern analysis has shown that meaB expression is not subject to nitrogen metabolite repression. meaB encodes a novel protein of 418 amino acids and contains a significantly high number of S/TPXX motifs, a motif common in transcriptional regulatory proteins. We have sequenced three mutations within meaB, two of which have an identical phenotype to that produced by gene disruption.
在构巢曲霉中，meaB基因内的突变引发了几种受氮代谢物阻遏的活性的不适当表达，并且还具有一些不相关的表型效应。我们已经克隆了meaB并分离出一个全长cDNA克隆。Northern分析表明，meaB的表达不受氮代谢物阻遏的影响。meaB编码一种由418个氨基酸组成的新型蛋白质，并且含有大量的S/TPXX基序，这是转录调节蛋白中常见的基序。我们已经对meaB内的三个突变进行了测序，其中两个与基因破坏产生的表型相同。
