自然微生物群落中高遗传多样性的原位可视化
In situ visualization of high genetic diversity in a natural microbial community.
作者信息
Amann R, Snaidr J, Wagner M, Ludwig W, Schleifer K H
机构信息
Lehrstuhl für Mikrobiologie, Technische Universität München, Germany.
出版信息
J Bacteriol. 1996 Jun;178(12):3496-500. doi: 10.1128/jb.178.12.3496-3500.1996.
Abstract
Simultaneous in situ visualization of seven distinct bacterial genotypes, all affiliated with the phylogenetically narrow group of beta-1 Proteobacteria, was achieved in activated sludge. This finding indicates that the high diversity found in the same sample by direct rRNA sequence retrieval was indeed present in this complex community. By the combination of comparative rRNA sequence analysis, in situ hybridization with fluorescently labeled, rRNA-targeted oligonucleotides and confocal laser scanning microscopy several microbial populations can be analyzed for abundance, relative spatial distribution and phylogeny directly at their site of action without prior cultivation.
摘要
在活性污泥中实现了对七种不同细菌基因型的同时原位可视化，所有这些基因型都隶属于系统发育关系较窄的β-1变形菌纲菌群。这一发现表明，通过直接rRNA序列检索在同一样本中发现的高度多样性确实存在于这个复杂的群落中。通过比较rRNA序列分析、与荧光标记的rRNA靶向寡核苷酸进行原位杂交以及共聚焦激光扫描显微镜技术的结合，可以直接在其作用位点分析几个微生物种群的丰度、相对空间分布和系统发育，而无需事先培养。
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