枯草芽孢杆菌和乳酸乳球菌中通过磷酸烯醇丙酮酸依赖性磷酸转移酶系统进行的糖摄取调节是由HPr中丝氨酸残基46的ATP依赖性磷酸化介导的。
Regulation of sugar uptake via the phosphoenolpyruvate-dependent phosphotransferase systems in Bacillus subtilis and Lactococcus lactis is mediated by ATP-dependent phosphorylation of seryl residue 46 in HPr.
作者信息
Ye J J, Saier M H
机构信息
Department of Biology, University of California at San Diego, La Jolla 92093-0116, USA.
出版信息
J Bacteriol. 1996 Jun;178(12):3557-63. doi: 10.1128/jb.178.12.3557-3563.1996.
Abstract
By using both metabolizable and nonmetabolizable sugar substrates of the phosphoenolpyruvate-dependent phosphotransferase system (PTS), we show that PTS sugar uptake into intact cells and membrane vesicles of Lactococcus lactis and Bacillus subtilis is strongly inhibited by high concentrations of any of several metabolizable PTS sugars. Inhibition requires phosphorylation of seryl residue 46 in the phosphocarrier protein of the PTS, HPr, by the metabolite-activated, ATP-dependent protein kinase. Inhibition does not occur when wild-type HPr is replaced by the S46A mutant form of this protein either in vesicles of L. lactis or B. subtilis or in intact cells of B. subtilis. Nonmetabolizable PTS sugar analogs such as 2-deoxyglucose inhibit PTS sugar uptake by a distinct mechanism that is independent of HPr(ser-P) and probably involves cellular phosphoenolpyruvate depletion.
摘要
通过使用磷酸烯醇丙酮酸依赖性磷酸转移酶系统（PTS）的可代谢和不可代谢糖底物，我们发现，高浓度的几种可代谢PTS糖中的任何一种都会强烈抑制乳酸乳球菌和枯草芽孢杆菌完整细胞及膜囊泡对PTS糖的摄取。这种抑制作用需要代谢物激活的、依赖ATP的蛋白激酶将PTS的磷酸载体蛋白HPr中的丝氨酸残基46磷酸化。当野生型HPr被该蛋白的S46A突变形式取代时，无论是在乳酸乳球菌或枯草芽孢杆菌的膜囊泡中，还是在枯草芽孢杆菌的完整细胞中，都不会发生抑制作用。不可代谢的PTS糖类似物，如2-脱氧葡萄糖，通过一种独立于HPr（丝氨酸-P）的独特机制抑制PTS糖的摄取，这可能涉及细胞内磷酸烯醇丙酮酸的消耗。
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Regulation of sugar uptake via the phosphoenolpyruvate-dependent phosphotransferase systems in Bacillus subtilis and Lactococcus lactis is mediated by ATP-dependent phosphorylation of seryl residue 46 in HPr.枯草芽孢杆菌和乳酸乳球菌中通过磷酸烯醇丙酮酸依赖性磷酸转移酶系统进行的糖摄取调节是由HPr中丝氨酸残基46的ATP依赖性磷酸化介导的。
J Bacteriol. 1996 Jun;178(12):3557-63. doi: 10.1128/jb.178.12.3557-3563.1996.
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本文引用的文献
1
Protein measurement with the Folin phenol reagent.使用福林酚试剂进行蛋白质测定。
J Biol Chem. 1951 Nov;193(1):265-75.
2
Catabolite repression and inducer control in Gram-positive bacteria.革兰氏阳性菌中的分解代谢物阻遏和诱导物控制
Microbiology (Reading). 1996 Feb;142 ( Pt 2):217-230. doi: 10.1099/13500872-142-2-217.
3
Inducer expulsion and the occurrence of an HPr(Ser-P)-activated sugar-phosphate phosphatase in Enterococcus faecalis and Streptococcus pyogenes.粪肠球菌和化脓性链球菌中诱导物排出及HPr(Ser-P)激活的糖磷酸磷酸酶的出现。
Microbiology (Reading). 1996 Mar;142 ( Pt 3):585-592. doi: 10.1099/13500872-142-3-585.
4
Identification of an anaerobically induced phosphoenolpyruvate-dependent fructose-specific phosphotransferase system and evidence for the Embden-Meyerhof glycolytic pathway in the heterofermentative bacterium Lactobacillus brevis.在兼性发酵细菌短乳杆菌中厌氧诱导的磷酸烯醇丙酮酸依赖性果糖特异性磷酸转移酶系统的鉴定及糖酵解途径的证据。
J Bacteriol. 1996 Jan;178(1):314-6. doi: 10.1128/jb.178.1.314-316.1996.
5
Catabolite repression of the Bacillus subtilis gnt operon mediated by the CcpA protein.由CcpA蛋白介导的枯草芽孢杆菌gnt操纵子的分解代谢物阻遏
J Bacteriol. 1994 Jan;176(2):511-3. doi: 10.1128/jb.176.2.511-513.1994.
6
Phosphoenolpyruvate:carbohydrate phosphotransferase systems of bacteria.细菌的磷酸烯醇丙酮酸：碳水化合物磷酸转移酶系统
Microbiol Rev. 1993 Sep;57(3):543-94. doi: 10.1128/mr.57.3.543-594.1993.
7
Regulation of the glucose:H+ symporter by metabolite-activated ATP-dependent phosphorylation of HPr in Lactobacillus brevis.短乳杆菌中通过代谢物激活的HPr的ATP依赖性磷酸化对葡萄糖:H⁺同向转运体的调控。
J Bacteriol. 1994 Jun;176(12):3484-92. doi: 10.1128/jb.176.12.3484-3492.1994.
8
Loss of protein kinase-catalyzed phosphorylation of HPr, a phosphocarrier protein of the phosphotransferase system, by mutation of the ptsH gene confers catabolite repression resistance to several catabolic genes of Bacillus subtilis.ptsH基因突变导致磷酸转移酶系统的磷酸载体蛋白HPr失去蛋白激酶催化的磷酸化作用，这使得枯草芽孢杆菌的几个分解代谢基因具有抗分解代谢物阻遏的特性。
J Bacteriol. 1994 Jun;176(11):3336-44. doi: 10.1128/jb.176.11.3336-3344.1994.
9
Inhibition of the phosphoenolpyruvate:lactose phosphotransferase system and activation of a cytoplasmic sugar-phosphate phosphatase in Lactococcus lactis by ATP-dependent metabolite-activated phosphorylation of serine 46 in the phosphocarrier protein HPr.通过磷酸载体蛋白HPr中丝氨酸46的ATP依赖性代谢物激活磷酸化作用，抑制乳酸乳球菌中的磷酸烯醇丙酮酸：乳糖磷酸转移酶系统并激活细胞质糖磷酸磷酸酶。
J Biol Chem. 1994 Apr 22;269(16):11837-44.
10
ATP-dependent phosphorylation of serine-46 in the phosphocarrier protein HPr regulates lactose/H+ symport in Lactobacillus brevis.磷酸载体蛋白HPr中丝氨酸-46的ATP依赖性磷酸化调节短乳杆菌中的乳糖/H⁺同向转运。
Proc Natl Acad Sci U S A. 1994 Apr 12;91(8):3102-6. doi: 10.1073/pnas.91.8.3102.
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