Reina J, Lopez A
Clinical Microbiology Service, Universitat Illes Balears (UIB), Palma de Mallorca, Spain.
J Clin Pathol. 1996 Feb;49(2):173-5. doi: 10.1136/jcp.49.2.173.
A case of acute diarrhoea caused by Aeromonas trota (formerly HG 13 group) in a Spanish child is reported. The strain was isolated in the faeces using the CIN agar (cefsulodin-irgasan-novobiocin) culture media. The strain was initially identified as A sobria by the commercial GNI card and API 20E biochemical systems. The strain was, however, VogesProskauer and sucrose negative, so complementary tests of cellobiose fermentation and gluconate oxidation were performed. These tests, together with the strain susceptibility to ampicillin (MIC 1 microgram/ml) and carbenicillin (MIC < 16 micrograms/ml) led to the final identification of A trota. The microbiological characteristics of this new species and the principal tests required for its identification are presented. The isolation, for the first time, of A trota in the Mediterranean area confirms the suspected worldwide distribution of this species.
报告了一名西班牙儿童由豚鼠气单胞菌(原HG 13组)引起的急性腹泻病例。使用CIN琼脂(头孢磺啶-伊红-新生霉素)培养基从粪便中分离出该菌株。该菌株最初通过商业GNI卡和API 20E生化系统鉴定为温和气单胞菌。然而,该菌株Voges-Proskauer试验和蔗糖试验呈阴性,因此进行了纤维二糖发酵和葡萄糖酸盐氧化的补充试验。这些试验,连同该菌株对氨苄西林(MIC为1微克/毫升)和羧苄青霉素(MIC < 16微克/毫升)的敏感性,最终鉴定为豚鼠气单胞菌。本文介绍了这个新物种的微生物学特征及其鉴定所需的主要试验。在地中海地区首次分离出豚鼠气单胞菌,证实了该物种在全球范围内的分布推测。