Seto Y
National Research Institute of Police Science, Tokyo, Japan.
J Forensic Sci. 1996 May;41(3):465-8.
To investigate the effects of heat on blood cyanide concentrations, in vitro experiments were performed using a headspace gas chromatographic method. Cyanide concentrations were determined for solutions of human hemoglobin (Hb) at neutrality, and for blood which was sealed in a vial and incubated at 25, 50, 63, 75 and 90 degrees C for 1 h. Spontaneous cyanide production was also measured. Nearly all of the added cyanide was recovered in both the Hb and for blood samples which were heated below 63 degrees C. Cyanide recovery in Hb decreased in a temperature-dependent manner at temperatures above 75 degrees C, and more than half of the recovered cyanide was found to be in the free form. In contrast, cyanide in blood disappeared more rapidly, and a major portion of it existed in the bound form. Cyanide concentrations in Hb solutions which were heated at 90 degrees C dropped in the two phases; a rapid initial phase, followed by a slower process. Spontaneous cyanide production was observed at temperatures above 50 degrees C for Hb and above 63 degrees C for blood. Under optimal conditions (75 degrees C heating), about 0.2 mmol of cyanide was produced per mol heme of Hb.
为研究加热对血液中氰化物浓度的影响，采用顶空气相色谱法进行了体外实验。测定了中性条件下人体血红蛋白（Hb）溶液以及密封于小瓶中并在25、50、63、75和90摄氏度下孵育1小时的血液中的氰化物浓度。还测量了自发产生的氰化物。在63摄氏度以下加热的Hb和血液样本中，几乎所有添加的氰化物都被回收。在75摄氏度以上，Hb中的氰化物回收率呈温度依赖性下降，且回收的氰化物中一半以上以游离形式存在。相比之下，血液中的氰化物消失得更快，且大部分以结合形式存在。在90摄氏度下加热的Hb溶液中的氰化物浓度分两个阶段下降：初始阶段迅速，随后过程较慢。在50摄氏度以上的Hb和63摄氏度以上的血液中观察到了自发氰化物产生。在最佳条件下（75摄氏度加热），每摩尔Hb血红素约产生0.2毫摩尔氰化物。
