通过流式细胞荧光测定法对淋巴细胞对PHA的反应进行定量分析。III.诱导期的异质性。
Quantitation of lymphocyte response to PHA by flow cytofluorometry. III. Heterogeneity of induction period.
作者信息
Braunstein J D, Schwartz G, Good R A, Sharpless T K, Melamed M R
出版信息
J Histochem Cytochem. 1979 Jan;27(1):474-7. doi: 10.1177/27.1.86571.
Early events in phytohaemagglutinin (PHA) stimulation of mouse splenocytes have been quantitated by using flow cytometry and supravital staining with acridine orange (AO). Increasing percentages of single cells with increased metachromatic (red) AO staining were demonstrated in cultures stimulated by PHA for up to 24 hr. These differences in staining could be eliminated by fixation with 1:1 ethanol/acetone before staining. Stimulated cells showed an increase in nonspecific esterase activity as measured by flow cytometry after supravital staining with fluorescein diacetate (FDA). The data reported show a heterogeneity in the per cell response of mouse splenocytes to PHA. The relationship between these data and the mechanism of mitogen stimulation is discussed.
通过流式细胞术和吖啶橙（AO）活体染色对植物血凝素（PHA）刺激小鼠脾细胞的早期事件进行了定量分析。在PHA刺激长达24小时的培养物中，显示出具有增多的异染性（红色）AO染色的单个细胞百分比不断增加。染色前用1:1乙醇/丙酮固定可消除这些染色差异。用荧光素二乙酸酯（FDA）活体染色后，通过流式细胞术测量发现，受刺激细胞的非特异性酯酶活性增加。所报道的数据显示小鼠脾细胞对PHA的单细胞反应存在异质性。讨论了这些数据与有丝分裂原刺激机制之间的关系。
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