Nicolini C, Belmont A, Parodi S, Lessin S, Abraham S
J Histochem Cytochem. 1979 Jan;27(1):102-13. doi: 10.1177/27.1.86559.
We present results involving an approach to acridine orange staining of intact cells based on basic physicochemical considerations. We show by static microfluorometry of several in vitro and in vivo cell lines that the important parameters for such staining are the molar ratio (Formula: see text), and molar concentration of acridine orange. Differential nuclear DNA and cytoplasmic RNA staining are totally controlled by these two parameters. We show this by a physicochemical model of cell-dye interaction. Finally, we use the method to study the growth parameters of complex in vivo cell populations by automated multiparameter flow microfluorometry. We have explored also, both by static and flow systems, the effect on AO-cell staining of various cell pretreatments such as Triton X-100 and chelating agents.
我们展示了基于基本物理化学考量的完整细胞吖啶橙染色方法的相关结果。通过对多种体外和体内细胞系进行静态显微荧光测定，我们发现这种染色的重要参数是吖啶橙的摩尔比（公式：见正文）和摩尔浓度。核DNA和细胞质RNA的差异染色完全由这两个参数控制。我们通过细胞 - 染料相互作用的物理化学模型证明了这一点。最后，我们使用该方法通过自动多参数流式显微荧光测定来研究体内复杂细胞群体的生长参数。我们还通过静态和流动系统探索了诸如Triton X - 100和螯合剂等各种细胞预处理对吖啶橙 - 细胞染色的影响。
