Schultz J S, Valentine R, Choi C Y
J Gen Physiol. 1979 Jan;73(1):49-60. doi: 10.1085/jgp.73.1.49.
Osmotic water flow through membranes with uniform defined pores was measured for a variety of macromolecular solutes. Water flow increased linearly with applied hydrostatic pressure, allowing the effective osmotic pressure of the solutes to be estimated by extrapolation. Reflection coefficients for each solute-membrane combination were calculated and correlated with the ratio of solute size to pore size. For the same mean molecular size, proteins were found to have larger reflection coefficients than dextrans. Molecular rigidity may play a role in this difference in behavior.
对多种大分子溶质，测量了通过具有均匀特定孔径膜的渗透水流。水流随施加的静水压力呈线性增加，从而可通过外推法估算溶质的有效渗透压。计算了每种溶质 - 膜组合的反射系数，并将其与溶质大小与孔径的比率相关联。对于相同的平均分子大小，发现蛋白质的反射系数比葡聚糖的大。分子刚性可能在这种行为差异中起作用。
