Diacovo T G, Puri K D, Warnock R A, Springer T A, von Andrian U H
Center for Blood Research, Harvard Medical School, 200 Longwood Avenue, Boston, MA 02115, USA.
Science. 1996 Jul 12;273(5272):252-5. doi: 10.1126/science.273.5272.252.
Circulating lymphocytes gain access to lymph nodes owing to their ability to initiate rolling along specialized high endothelial venules (HEVs). One mechanism of rolling involves L-selectin binding to peripheral node addressin (PNAd) on HEVs. Activated platelets are shown to bind to circulating lymphocytes and to mediate rolling in HEVs, in vivo, through another molecule, P-selectin, which also interacts with PNAd. In vitro, activated platelets enhanced tethering of lymphocytes to PNAd and sustained lymphocyte rolling, even in the absence of functional L-selectin. Thus, a platelet pathway operating through P-selectin provides a second mechanism for lymphocyte delivery to HEVs.
循环淋巴细胞能够沿着特殊的高内皮微静脉（HEV）起始滚动，从而进入淋巴结。滚动的一种机制涉及L-选择素与HEV上的外周淋巴结地址素（PNAd）结合。在体内，活化的血小板可与循环淋巴细胞结合，并通过另一种分子P-选择素介导淋巴细胞在HEV中的滚动，P-选择素也与PNAd相互作用。在体外，即使在缺乏功能性L-选择素的情况下，活化的血小板也能增强淋巴细胞与PNAd的黏附并维持淋巴细胞滚动。因此，通过P-选择素发挥作用的血小板途径为淋巴细胞输送至HEV提供了第二种机制。
