Apollonin A V, Volchkova E V, Dmitrieva E V, Iakovlev M Iu, Malov V A, Pak S G, Likhoded V G
Vestn Ross Akad Med Nauk. 1995(12):38-41.
Bacterial endotoxins (BE) that are lipopolysaccharide complexes (LPS) are a structural component of the external membrane of gram-negative bacteria. In normalcy, BE interact with many types of cells in the mammals. In terms of the concentration, BE may cause cell damage or stimulate the production of many biological mediators, such as interleukins, prostaglandinds, alpha-TNF. Many gastrointestinal bacteria in humans are gram-negative and BE constantly enter the blood. In health, the absence of a toxic response to BE is explained by the presence of natural humoral and cellular antiendotoxic systems and the hepatic absorption of LPS. In patients with hepatitis A and B, the following indices of the blood antiendotoxic systems were determined: the level of antiendotoxic antibodies to Re-chemotype glycolipids was assessed by the passive hemagglutination reaction in the "Antiendotox-1-test"; the count of polymorphonuclear leukocyte (PMNL) fixating LPS on their own surface and endotoxin binding function of PMNL was in vitro measured by the strain ELISA and sandwich ELISA with Re-glycolipids, respectively (LPS-test); the endotoxin fixation function of serum high density lipoproteins (HDL) was also assessed. The humoral and cellular antiendotoxic systems in patients with mild advanced hepatitis A and B was studied when the disease was most clinically significant, at an early convalescence, and at convalescence itself. Finally, the findings indicate that there is a significant decrease in Re-antibody levels and there is a greater absorption ability of HDL than that in the control. Six different types of an antiendotoxic fixation reaction of PMNL were identified in patients with viral hepatitis in the different periods of the disease. The alterations observed may play an important role in the pathogenesis of toxemia in patients with viral hepatitis.
细菌内毒素（BE）是脂多糖复合物（LPS），是革兰氏阴性菌外膜的结构成分。正常情况下，BE与哺乳动物的多种细胞相互作用。就浓度而言，BE可能导致细胞损伤或刺激多种生物介质的产生，如白细胞介素、前列腺素、α -肿瘤坏死因子。人类许多胃肠道细菌为革兰氏阴性菌，BE不断进入血液。在健康状态下，对BE无毒性反应是由于存在天然的体液和细胞抗内毒素系统以及肝脏对LPS的吸收。对甲型和乙型肝炎患者，测定了血液抗内毒素系统的以下指标：通过“抗内毒素 - 1测试”中的被动血凝反应评估针对Re - 化学型糖脂的抗内毒素抗体水平；分别通过菌株ELISA和用Re - 糖脂的夹心ELISA体外测量多形核白细胞（PMNL）在其自身表面固定LPS的数量以及PMNL的内毒素结合功能（LPS测试）；还评估了血清高密度脂蛋白（HDL）的内毒素固定功能。在甲型和乙型肝炎轻度进展期患者疾病最具临床意义时、早期恢复期以及恢复期本身，研究了其体液和细胞抗内毒素系统。最后，研究结果表明Re - 抗体水平显著降低，且HDL的吸收能力比对照组更强。在病毒性肝炎患者疾病的不同时期，鉴定出六种不同类型的PMNL抗内毒素固定反应。观察到的这些改变可能在病毒性肝炎患者毒血症的发病机制中起重要作用。
