• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

哺乳动物聚腺苷酸聚合酶的突变分析确定了一个与X家族聚合酶及其他核苷酸转移酶同源的引物结合区域和催化结构域。

Mutational analysis of mammalian poly(A) polymerase identifies a region for primer binding and catalytic domain, homologous to the family X polymerases, and to other nucleotidyltransferases.

作者信息

Martin G, Keller W

机构信息

Department of Cell Biology, University of Basel, Basel, Switzerland.

出版信息

EMBO J. 1996 May 15;15(10):2593-603.

PMID:8665867
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC450192/
Abstract

We have tested deletion and substitution mutants of bovine poly(A) polymerase, and have identified a small region that overlaps with a nuclear localization signal and binds to the RNA primer. Systematic mutagenesis of carboxylic amino acids led to the identification of three aspartates that are essential for catalysis. Sequence and secondary structure comparisons of regions surrounding these aspartates with sequences of other polymerases revealed a significant homology to the palm structure of DNA polymerase beta, terminal deoxynucleotidyltransferase and DNA polymerase IV of Saccharomyces cerevisiae, all members of the family X of polymerases. This homology extends as far as cca: tRNA nucleotidyltransferase and streptomycin adenylyltransferase, an antibiotic resistance factor.

摘要

我们对牛多聚腺苷酸聚合酶的缺失和替代突变体进行了测试，并确定了一个与核定位信号重叠且能与RNA引物结合的小区域。对羧基氨基酸进行系统诱变后，鉴定出了三个对催化作用至关重要的天冬氨酸。将这些天冬氨酸周围区域的序列和二级结构与其他聚合酶的序列进行比较，发现其与DNA聚合酶β、末端脱氧核苷酸转移酶以及酿酒酵母DNA聚合酶IV（均为X家族聚合酶成员）的掌状结构具有显著同源性。这种同源性一直延伸到cca:tRNA核苷酸转移酶和链霉素腺苷酸转移酶（一种抗生素抗性因子）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab13/450192/ace0a246abcf/emboj00010-0280-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab13/450192/a5140f718391/emboj00010-0274-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab13/450192/ffa984242121/emboj00010-0275-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab13/450192/62342c17362a/emboj00010-0276-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab13/450192/1b92f74b5a53/emboj00010-0276-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab13/450192/5b2f98aab95c/emboj00010-0277-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab13/450192/a1d7ea9e382c/emboj00010-0278-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab13/450192/ace0a246abcf/emboj00010-0280-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab13/450192/a5140f718391/emboj00010-0274-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab13/450192/ffa984242121/emboj00010-0275-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab13/450192/62342c17362a/emboj00010-0276-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab13/450192/1b92f74b5a53/emboj00010-0276-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab13/450192/5b2f98aab95c/emboj00010-0277-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab13/450192/a1d7ea9e382c/emboj00010-0278-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/ab13/450192/ace0a246abcf/emboj00010-0280-a.jpg

相似文献

1
Mutational analysis of mammalian poly(A) polymerase identifies a region for primer binding and catalytic domain, homologous to the family X polymerases, and to other nucleotidyltransferases.哺乳动物聚腺苷酸聚合酶的突变分析确定了一个与X家族聚合酶及其他核苷酸转移酶同源的引物结合区域和催化结构域。
EMBO J. 1996 May 15;15(10):2593-603.
2
Mapping of ATP binding regions in poly(A) polymerases by photoaffinity labeling and by mutational analysis identifies a domain conserved in many nucleotidyltransferases.通过光亲和标记和突变分析对聚腺苷酸聚合酶中ATP结合区域进行定位，确定了在许多核苷酸转移酶中保守的一个结构域。
Protein Sci. 1999 Nov;8(11):2380-91. doi: 10.1110/ps.8.11.2380.
3
Arg304 of human DNA primase is a key contributor to catalysis and NTP binding: primase and the family X polymerases share significant sequence homology.人类DNA引发酶的第304位精氨酸是催化作用和三磷酸核苷结合的关键因素：引发酶与X家族聚合酶具有显著的序列同源性。
Biochemistry. 1999 Jun 15;38(24):7727-36. doi: 10.1021/bi990247c.
4
A family of poly(U) polymerases.一个聚尿苷酸聚合酶家族。
RNA. 2007 Jun;13(6):860-7. doi: 10.1261/rna.514007. Epub 2007 Apr 20.
5
Crystal structure of a pol alpha family DNA polymerase from the hyperthermophilic archaeon Thermococcus sp. 9 degrees N-7.嗜热古菌嗜热栖热菌9°N - 7来源的聚合酶α家族DNA聚合酶的晶体结构
J Mol Biol. 2000 Jun 2;299(2):447-62. doi: 10.1006/jmbi.2000.3728.
6
Crystal structures of human DNA polymerase beta complexed with DNA: implications for catalytic mechanism, processivity, and fidelity.与DNA复合的人类DNA聚合酶β的晶体结构：对催化机制、持续合成能力和保真度的影响
Biochemistry. 1996 Oct 1;35(39):12742-61. doi: 10.1021/bi952955d.
7
A regulatory cytoplasmic poly(A) polymerase in Caenorhabditis elegans.秀丽隐杆线虫中的一种调节性细胞质聚腺苷酸聚合酶。
Nature. 2002 Sep 19;419(6904):312-6. doi: 10.1038/nature01039.
8
Distinct roles of two Yth1p domains in 3'-end cleavage and polyadenylation of yeast pre-mRNAs.酵母前体mRNA 3' 端切割和聚腺苷酸化过程中Yth1p蛋白两个结构域的不同作用
EMBO J. 2000 Jul 17;19(14):3778-87. doi: 10.1093/emboj/19.14.3778.
9
Structural modeling identified the tRNA-binding domain of Utp8p, an essential nucleolar component of the nuclear tRNA export machinery of Saccharomyces cerevisiae.结构建模确定了Utp8p的tRNA结合结构域，Utp8p是酿酒酵母细胞核tRNA输出机制中一个必需的核仁组分。
Biochem Cell Biol. 2009 Apr;87(2):431-43. doi: 10.1139/o08-145.
10
Biochemical and structural insights into substrate binding and catalytic mechanism of mammalian poly(A) polymerase.对哺乳动物多聚腺苷酸聚合酶底物结合和催化机制的生化及结构见解。
J Mol Biol. 2004 Aug 20;341(4):911-25. doi: 10.1016/j.jmb.2004.06.047.

引用本文的文献

1
The polyadenylase PAPI is required for virulence plasmid maintenance in pathogenic bacteria.多聚腺苷酸酶PAPI是致病细菌中毒力质粒维持所必需的。
PLoS Pathog. 2025 May 27;21(5):e1012655. doi: 10.1371/journal.ppat.1012655. eCollection 2025 May.
2
The polyadenylase PAPI is required for virulence plasmid maintenance in pathogenic bacteria.多聚腺苷酸酶PAPI是致病细菌中毒力质粒维持所必需的。
bioRxiv. 2024 Nov 8:2024.10.11.617751. doi: 10.1101/2024.10.11.617751.
3
Molecular basis of human poly(A) polymerase recruitment by mPSF.人多聚腺苷酸聚合酶被 mPSF 招募的分子基础。

本文引用的文献

1
Assembly of a processive messenger RNA polyadenylation complex.进行性信使核糖核酸聚腺苷酸化复合体的组装
EMBO J. 1993 Feb;12(2):585-94. doi: 10.1002/j.1460-2075.1993.tb05690.x.
2
Mammalian poly(A)-binding protein II. Physical properties and binding to polynucleotides.哺乳动物聚腺苷酸结合蛋白II。物理性质及与多核苷酸的结合
J Biol Chem. 1993 Feb 5;268(4):2937-45.
3
The KH domain occurs in a diverse set of RNA-binding proteins that include the antiterminator NusA and is probably involved in binding to nucleic acid.KH结构域存在于多种RNA结合蛋白中，包括抗终止因子NusA，可能参与与核酸的结合。
RNA. 2024 Jun 17;30(7):795-806. doi: 10.1261/rna.079915.123.
4
Depletion of tRNA CCA-adding enzyme in Mycobacterium tuberculosis leads to polyadenylation of transcripts and precursor tRNAs.结核分枝杆菌中 tRNA CCA- 添加酶的缺失导致转录物和前体 tRNA 的多聚腺苷酸化。
Sci Rep. 2023 Nov 24;13(1):20717. doi: 10.1038/s41598-023-47944-6.
5
Tyrosine phosphorylation controlled poly(A) polymerase I activity regulates general stress response in bacteria.酪氨酸磷酸化控制多聚（A）聚合酶 I 活性调节细菌的一般应激反应。
Life Sci Alliance. 2022 Dec 19;6(3). doi: 10.26508/lsa.202101148. Print 2023 Mar.
6
CRISPR/Cas9-mediated mutation of tyrosinase (Tyr) 3' UTR induce graying in rabbit.CRISPR/Cas9 介导的酪氨酸酶 (Tyr) 3'UTR 突变诱导家兔毛发变白。
Sci Rep. 2017 May 8;7(1):1569. doi: 10.1038/s41598-017-01727-y.
7
The nuclear poly(A) binding protein of mammals, but not of fission yeast, participates in mRNA polyadenylation.哺乳动物而非裂殖酵母的细胞核多聚腺苷酸结合蛋白参与mRNA的多聚腺苷酸化过程。
RNA. 2017 Apr;23(4):473-482. doi: 10.1261/rna.057026.116. Epub 2017 Jan 17.
8
An investigation into the role of ATP in the mammalian pre-mRNA 3' cleavage reaction.关于ATP在哺乳动物前体mRNA 3' 切割反应中作用的研究。
Biochimie. 2016 Jun;125:213-22. doi: 10.1016/j.biochi.2016.04.004. Epub 2016 Apr 6.
9
The polyadenylation complex of Trypanosoma brucei: Characterization of the functional poly(A) polymerase.布氏锥虫的聚腺苷酸化复合体：功能性聚（A）聚合酶的特性
RNA Biol. 2016;13(2):221-31. doi: 10.1080/15476286.2015.1130208.
10
Polyuridylation in Eukaryotes: A 3'-End Modification Regulating RNA Life.真核生物中的多聚尿苷酸化：一种调控RNA寿命的3'末端修饰
Biomed Res Int. 2015;2015:968127. doi: 10.1155/2015/968127. Epub 2015 May 11.
FEBS Lett. 1993 Jun 21;324(3):361-6. doi: 10.1016/0014-5793(93)80152-k.
4
Molecular genetics of aminoglycoside resistance genes and familial relationships of the aminoglycoside-modifying enzymes.氨基糖苷类耐药基因的分子遗传学及氨基糖苷类修饰酶的家族关系。
Microbiol Rev. 1993 Mar;57(1):138-63. doi: 10.1128/mr.57.1.138-163.1993.
5
Prediction of protein secondary structure at better than 70% accuracy.蛋白质二级结构预测准确率高于70%。
J Mol Biol. 1993 Jul 20;232(2):584-99. doi: 10.1006/jmbi.1993.1413.
6
The SWISS-PROT protein sequence data bank, recent developments.SWISS-PROT蛋白质序列数据库，最新进展。
Nucleic Acids Res. 1993 Jul 1;21(13):3093-6. doi: 10.1093/nar/21.13.3093.
7
The human U1A snRNP protein regulates polyadenylation via a direct interaction with poly(A) polymerase.人类U1A小核核糖核蛋白（snRNP）通过与聚腺苷酸聚合酶直接相互作用来调节聚腺苷酸化。
Cell. 1994 Feb 11;76(3):531-41. doi: 10.1016/0092-8674(94)90116-3.
8
Multiple forms of poly(A) polymerases in human cells.人类细胞中多种形式的聚腺苷酸聚合酶
Proc Natl Acad Sci U S A. 1994 Feb 1;91(3):979-83. doi: 10.1073/pnas.91.3.979.
9
Analysis of the RNA-recognition motif and RS and RGG domains: conservation in metazoan pre-mRNA splicing factors.RNA识别基序以及RS和RGG结构域的分析：后生动物前体mRNA剪接因子中的保守性
Nucleic Acids Res. 1993 Dec 25;21(25):5803-16. doi: 10.1093/nar/21.25.5803.
10
Yeast open reading frame YCR14C encodes a DNA beta-polymerase-like enzyme.酵母开放阅读框YCR14C编码一种DNAβ聚合酶样酶。
Nucleic Acids Res. 1993 Nov 25;21(23):5301-7. doi: 10.1093/nar/21.23.5301.