Benavente Micaela Andrea, Bianchi Carolina Paula, Imperiale Fernanda, Aba Marcelo Alfredo
Laboratorio de Endocrinología, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA) , Tandil , Argentina.
Laboratorio de Farmacología, Centro de Investigación Veterinaria de Tandil (CIVETAN), CONICET, Facultad de Ciencias Veterinarias, Universidad Nacional del Centro de la Provincia de Buenos Aires (UNCPBA) , Tandil , Argentina.
Front Vet Sci. 2016 Dec 26;3:119. doi: 10.3389/fvets.2016.00119. eCollection 2016.
Neoplasms of the mammary gland represent the most frequent tumor type in the female dog, and according to the histologic criteria, approximately 50% of them are malignant. In the most aggressive cases of mammary cancer, surgery is not enough to warrant a favorable outcome, and adjuvant therapies are needed to improve the patient's overall survival. The aim of the present study was to evaluate the effects of two peptides on proliferation of a canine mammary cancer cell line derived from a simple carcinoma. The cell line CMT-U27 was grown in 96-well plates, at two cell densities (4 × 10 and 8 × 10 cells/well). Cultures were treated with oxytocin (OT) or desmopressin at five concentrations (10, 50, 100, 500, and 1000 nM). After 72 h of incubation, cell proliferation was determined by the MTT assay. Results showed that with 4 × 10 cells/well, OT at 50, 500, and 1000 nM was growth inhibitory for the cells, being statistically significant at 1000 nM. On the contrary, no antiproliferative effect was observed with 10 or 100 nM. At 8 × 10 cells/well, OT showed a significant antiproliferative effect only with the highest concentration (1000 nM). Desmopressin at 4 × 10 cells/well decreased cell viability at concentrations of 50, 100, 500, and 1000 nM (statistically significant with the highest concentration), while no effect was observed with 10 nM. With 8 × 10 cells/well, this peptide reduced cell growth at 100, 500, and 1000 nM. In conclusion, we suggest that these peptides may be potential and promising compounds for the treatment of dogs with simple carcinomas of the mammary gland. studies are required to confirm this hypothesis.
乳腺肿瘤是雌性犬类中最常见的肿瘤类型，根据组织学标准，其中约50%为恶性肿瘤。在最具侵袭性的乳腺癌病例中，手术不足以保证良好的预后，需要辅助治疗来提高患者的总体生存率。本研究的目的是评估两种肽对源自单纯癌的犬乳腺癌细胞系增殖的影响。将CMT-U27细胞系以两种细胞密度（4×10和8×10个细胞/孔）接种于96孔板中。培养物用催产素（OT）或去氨加压素以五种浓度（10、50、100、500和1000 nM）处理。孵育72小时后，通过MTT法测定细胞增殖。结果显示，当细胞密度为4×10个细胞/孔时，50、500和1000 nM的OT对细胞具有生长抑制作用，在1000 nM时具有统计学意义。相反，10或100 nM未观察到抗增殖作用。当细胞密度为8×10个细胞/孔时，OT仅在最高浓度（1000 nM）时显示出显著的抗增殖作用。当细胞密度为4×10个细胞/孔时，50、100、500和1000 nM的去氨加压素降低了细胞活力（在最高浓度时具有统计学意义），而10 nM未观察到作用。当细胞密度为8×10个细胞/孔时，该肽在100、500和1000 nM时降低了细胞生长。总之，我们认为这些肽可能是治疗患有乳腺单纯癌的犬类的潜在且有前景的化合物。需要进一步研究来证实这一假设。
