Mainstone J C, Bruce A S, Golding T R
Centre for Eye Research, School of Optometry, Queensland University of Technology, Locked Bag No. 2, Red Hill, Queensland, 4029, Australia.
Curr Eye Res. 1996 Jun;15(6):653-61. doi: 10.3109/02713689609008906.
Assessment of the tear film meniscus is a quantitative, minimally invasive, direct measurement of tear film quantity. The aim of this study was to assess the efficacy of tear meniscus parameter measurement in the diagnosis of dry eye.
Tear meniscus radius of curvature, height, width and cross-sectional area (TMC, TMH, TMW, XSA) were determined by photographing an optic section of the inferior tear meniscus (colored with a min volume of fluorescein) at 12 x magnification, and then scanning developed images into a computer analysis program. Fifteen dry eye subjects and 15 age-matched controls were assessed. Dry eye subjects satisfied the criteria of a rose bengal staining score >/= 1, and a mean phenol red thread 15s wetted length </= 10 mm.
TMC, TMH and XSA were all reduced in magnitude in the dry eye group compared to the control group (mean +/- SD; TMC: 0.314 +/- 0.160 mm vs. 0.545 +/- 0.259 mm, TMH: 0.244 +/- 0.089 mm vs. 0.461 +/- 00.173 mm, XSA: 0.0082 +/- 0.0048 mm2 vs. 0.0176 +/- 0.0103 mm2, ANOVA, p < 0.05). Both TMC and TMH showed good diagnostic accuracy (166.7% and 160% respectively), with a dry eye referent value of </=0.35 mm for each parameter. TMC and TMH also showed strong correlations with the cotton thread test, non-invasive breakup time, and ocular surface staining scores (p < 0.01). TMH was the most powerful predictor of tear film insufficiency.
This study has shown tear meniscus assessment to be a useful alternative to existing tests for dry eye.
泪膜半月皱襞评估是一种对泪膜量进行定量、微创的直接测量方法。本研究的目的是评估泪膜半月皱襞参数测量在干眼诊断中的有效性。
通过以12倍放大率拍摄下方泪膜半月皱襞（用最小体积的荧光素染色）的光学切片，然后将显影后的图像扫描到计算机分析程序中，来测定泪膜半月皱襞的曲率半径、高度、宽度和横截面积（TMC、TMH、TMW、XSA）。评估了15名干眼受试者和15名年龄匹配的对照者。干眼受试者符合孟加拉玫瑰红染色评分≥1且酚红棉线平均湿润长度≤10 mm的标准。
与对照组相比，干眼组的TMC、TMH和XSA在数值上均降低（均值±标准差；TMC：0.314±0.160 mm对0.545±0.259 mm，TMH：0.244±0.089 mm对0.461±0.173 mm，XSA：0.0082±0.0048 mm²对0.0176±0.0103 mm²，方差分析，p<0.05）。TMC和TMH均显示出良好的诊断准确性（分别为166.7%和160%），每个参数的干眼参考值≤0.35 mm。TMC和TMH还与棉线试验、非侵入性泪膜破裂时间和眼表染色评分密切相关（p<0.01）。TMH是泪膜不足的最有力预测指标。
本研究表明泪膜半月皱襞评估是现有干眼检测方法的一种有用替代方法。
