Adam T, Giry M, Boquet P, Sansonetti P
Unité de Pathogénie Microbienne Moléculaire, INSERM U389, Paris, France.
EMBO J. 1996 Jul 1;15(13):3315-21.
The small GTPase rho is functionally involved in the formation of cytoskeletal structures like stress fibers or focal adhesion plaques. Shigella entry into HeLa cells induces a blossom-like membrane structure at the bacterial entry site. We show here that this membrane-folding process is rho-dependent. The three rho isoforms were recruited into bacterial entry sites with differential localization relative to the membrane structure. A rho-specific inhibitor abolished Shigella-induced membrane folding and impaired bacterial entry accordingly. S1-myosin labeling indicated that rho was involved in Shigella-induced actin polymerization but not actin nucleation in the bacterial invasion site. This provides a major link in the signalization cascade allowing entry of a bacterial pathogen into a eukaryotic cell.
小GTP酶rho在功能上参与细胞骨架结构的形成，如应力纤维或粘着斑。志贺氏菌进入HeLa细胞会在细菌进入位点诱导出一种花状膜结构。我们在此表明，这种膜折叠过程依赖于rho。三种rho亚型被招募到细菌进入位点，相对于膜结构具有不同的定位。一种rho特异性抑制剂消除了志贺氏菌诱导的膜折叠，并相应地损害了细菌的进入。S1-肌球蛋白标记表明rho参与了志贺氏菌诱导的细菌入侵位点的肌动蛋白聚合，但不参与肌动蛋白成核。这在信号级联反应中提供了一个主要环节，使细菌病原体能够进入真核细胞。
