Wang N, Shaulsky G, Escalante R, Loomis W F
Center for Molecular Genetics, Department of Biology, University of California at San Diego, La Jolla, CA 92093, USA.
EMBO J. 1996 Aug 1;15(15):3890-8.
A mutant which failed to complete development was isolated from a population of cells that had been subjected to insertional mutagenesis using restriction enzyme-mediated integration. The disrupted gene, dhkA, encodes the conserved motifs of a histidine kinase as well as the response regulator domain. It is likely that the histidine in DhkA is autophosphorylated and the phosphate passed to one or more response regulators. Such two-component systems function in a variety of bacterial signal transduction pathways and have been characterized recently in yeast and Arabidopsis. In Dictyostelium, we found that DhkA functions both in the regulation of prestalk gene expression and in the control of the terminal differentiation of prespore cells.
从一群经限制性内切酶介导整合进行插入诱变的细胞中分离出一个未能完成发育的突变体。被破坏的基因dhkA编码组氨酸激酶的保守基序以及应答调节结构域。DhkA中的组氨酸很可能发生自身磷酸化，然后磷酸基团传递给一个或多个应答调节因子。这种双组分系统在多种细菌信号转导途径中发挥作用，最近在酵母和拟南芥中也有相关研究。在盘基网柄菌中，我们发现DhkA在柄细胞前体基因表达的调控以及前孢子细胞终末分化的控制中均发挥作用。
