Schuster S C, Noegel A A, Oehme F, Gerisch G, Simon M I
Abteilung fur Membranebiochemie, Max-Planck-Institut für Biochemie, 82152 Martinsried, Germany.
EMBO J. 1996 Aug 1;15(15):3880-9.
We have used PCR to identify a Dictyostelium homolog of the bacterial two-component system. The gene dokA codes for a member of the hybrid histidine kinase family which is defined by the presence of conserved amino acid sequence motifs corresponding to an N-terminal receptor domain, a central kinase and a C-terminal response regulator moiety. Potential function of the regulator domain was demonstrated by phosphorylation in vitro. dokA mutants are deficient in the osmoregulatory pathway, resulting in premature cell death under high osmotic stress. Under less stringent osmotic conditions, cells grow at a normal rate, but development at the multicellular stage is altered. dokA is a member of a family of histidine kinase-like genes that play regulatory roles in eukaryotic cell function.
我们利用聚合酶链反应（PCR）鉴定出了一种与细菌双组分系统同源的盘基网柄菌基因。基因dokA编码一种杂合组氨酸激酶家族成员，该家族的定义是存在与N端受体结构域、中央激酶和C端响应调节部分相对应的保守氨基酸序列基序。调节结构域的潜在功能通过体外磷酸化得以证明。dokA突变体在渗透调节途径中存在缺陷，导致在高渗透压胁迫下细胞过早死亡。在不太严格的渗透条件下，细胞以正常速率生长，但在多细胞阶段的发育会发生改变。dokA是一组在真核细胞功能中起调节作用的组氨酸激酶样基因家族的成员。
