Eggert-Kruse W, Rohr G, Kerbel H, Schwalbach B, Demirakca T, Klinga K, Tilgen W, Runnebaum B
Department of Gynecological Endocrinology and Reproductive Medicine, Women's Hospital, Heidelberg, Germany.
Hum Reprod. 1996 Apr;11(4):784-9. doi: 10.1093/oxfordjournals.humrep.a019255.
To determine the clinical usefulness of Acridine Orange (AO) staining of spermatozoa as a screening test for the evaluation of semen quality during basic infertility investigation, semen smears from 103 randomly chosen males of subfertile couples were examined. The median duration of infertility was 4.5 years (range 1-15) and the median age was 33 years (range 21-43). The outcome of AO staining ranged from 5 to 81%, with a median of 24%, green fluorescent spermatozoa. Results were not significantly related to the parameters of semen analysis (sperm count, motility, standard morphology, viability, pH and volume, as well as fructose concentration and number of found cells) or to local sperm antibody testing and semen cultures. Fluorescence after AO staining was also not related to sperm functional capacity (evaluated using sperm-mucus interaction tests in vitro and in vivo), or the medical history of the patient. No significant differences in the AO test outcome were seen in patients with explained and unexplained infertility, or with regard to subsequent fertility [with a median value of 21% (range 5-46) green fluorescence in the fertile group, compared with a median value of 28% (range 9-81) green fluorescence in the other men]. The results of this prospective study indicate that under the usual conditions of conception, the AO test is not clinically useful as a screening procedure to determine semen quality during basic infertility investigation.
为了确定吖啶橙（AO）染色精子作为基础不孕症检查中精液质量评估筛查试验的临床实用性，对103对随机选择的不育夫妇中男性的精液涂片进行了检查。不育的中位持续时间为4.5年（范围1 - 15年），中位年龄为33岁（范围21 - 43岁）。AO染色结果为5%至81%，绿色荧光精子的中位数为24%。结果与精液分析参数（精子计数、活力、标准形态、存活率、pH值和体积，以及果糖浓度和发现的细胞数量）、局部精子抗体检测和精液培养均无显著相关性。AO染色后的荧光也与精子功能能力（通过体外和体内精子 - 黏液相互作用试验评估）或患者病史无关。在有明确病因和不明病因的不育患者中，或在后续生育情况方面，AO试验结果无显著差异[生育组绿色荧光的中位数为21%（范围5% - 46%），其他男性为28%（范围9% - 81%）]。这项前瞻性研究的结果表明，在通常的受孕条件下，AO试验作为基础不孕症检查中确定精液质量的筛查程序在临床上并无用处。
