Hoshi K, Katayose H, Yanagida K, Kimura Y, Sato A
Department of Obstetrics and Gynecology, Fukushima Medical College, Japan.
Fertil Steril. 1996 Oct;66(4):634-9. doi: 10.1016/s0015-0282(16)58581-1.
To determine whether the outcome of IVF can be predicted by acridine orange (AO) nuclear fluorescence of sperm.
Based on the fact that AO nuclear fluorescence color after acid treatment reflects maturity (green fluorescence) or immaturity (yellow to red fluorescence) of spermatozoa, the relationships between sperm maturity and the outcome of IVF, subzonal insemination, or intracytoplasmic sperm injection (ICSI) were investigated.
The IVF program at the Obstetrics and Gynecology Hospital, Fukushima Medical College.
Sixty-eight patients undergoing 68 IVF treatment cycles.
Acridine orange fluorescence of sperm nuclei and successful oocyte fertilization.
conventional semen parameters (sperm concentration and percentages of motile or morphologically normal spermatozoa in semen) did not correlate with the incidence of spermatozoa with green AO fluorescent (mature) nuclei. When > or = 50% of spermatozoa in semen samples exhibited green AO nuclear fluorescence, IVF was always successful. When green AO nuclear fluorescence was < 50%, only 39% of IVF treatment cycles (13/33) were successful. Only green AO fluorescent spermatozoa were able to bind efficiently human zona pellucida. When the incidence of green AO fluorescent spermatozoa was < 50%, no pregnancy resulted even though an average of 26% of the oocytes could be fertilized by ICSI.
The spermatozoa which fertilized oocytes in vivo and in IVF were limited to those whose nuclei exhibited green AO fluorescence. Intracytoplasmic sperm injection may be the method of choice when the incidence of green AO nuclear fluorescence is low regardless of the results of semen analysis.
确定精子的吖啶橙（AO）核荧光能否预测体外受精（IVF）的结果。
基于酸处理后AO核荧光颜色反映精子成熟度（绿色荧光）或未成熟度（黄色至红色荧光）这一事实，研究精子成熟度与IVF、透明带下授精或卵胞浆内单精子注射（ICSI）结果之间的关系。
福岛医学院妇产科医院的IVF项目。
68例接受68个IVF治疗周期的患者。
精子细胞核的吖啶橙荧光和卵母细胞成功受精情况。
传统精液参数（精液中精子浓度以及活动精子或形态正常精子的百分比）与具有绿色AO荧光（成熟）细胞核的精子发生率无关。当精液样本中≥50%的精子表现出绿色AO核荧光时，IVF总是成功的。当绿色AO核荧光＜50%时，仅39%的IVF治疗周期（13/33）成功。只有绿色AO荧光精子能够有效结合人透明带。当绿色AO荧光精子的发生率＜50%时，即使平均26%的卵母细胞可通过ICSI受精，也未发生妊娠。
在体内和IVF中使卵母细胞受精的精子仅限于细胞核呈现绿色AO荧光的精子。当绿色AO核荧光发生率较低时，无论精液分析结果如何，卵胞浆内单精子注射可能是首选方法。
