Nybroe O, Einarson K, Ahl T
Department of Ecology and Molecular Biology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.
Lett Appl Microbiol. 1996 May;22(5):366-70. doi: 10.1111/j.1472-765x.1996.tb01180.x.
Growth and viability of Alcaligenes eutrophus JMP134 was studied in laboratory microcosms with 0.2 microns-filtered seawater prior to release in field-based mesocosms. In unamended systems JMP134 did not grow and viability, measured as direct viable counts combined with immunofluorescence microscopy, was 40-50%. Addition of a nitrogen + phosphorus nutrient mixture caused a greater growth response than amendment with a carbon substrate. Amendment with substrate and/or nutrients caused an increase in viability to ca 100% but only for a brief period coinciding with cell proliferation. Hence, Alc. eutrophus JMP134 has a limited survival potential in seawater unless it is supplied with additional nutrients.
在将嗜中温产碱菌JMP134投放到野外中宇宙之前，先在实验室微观世界中，使用经0.2微米过滤的海水对其生长和生存能力进行了研究。在未改良的系统中，JMP134无法生长，通过直接活菌计数结合免疫荧光显微镜测量的生存能力为40%-50%。添加氮+磷营养混合物比添加碳底物改良剂引起的生长反应更大。用底物和/或营养物改良会使生存能力提高到约100%，但仅在与细胞增殖同时发生的短时间内如此。因此，除非提供额外的营养物质，嗜中温产碱菌JMP134在海水中的生存潜力有限。
