Repaske R, Repaske A C
Appl Environ Microbiol. 1976 Oct;32(4):585-91. doi: 10.1128/aem.32.4.585-591.1976.
Quantitative nutrient requirements for unrestricted autotrophic growth of Alcaligenes eutrophus were determined. Minimum saturating concentrations of Mg2+, SO42-, PO43-, Fe3+, and Na2+ for an optical density increase of 2 were 10(-4) M 8 X10(-5) M, 5 X 10(-4) to 6 X 10(-4) M, 10(-5) M, and 10(-7) to 2 X 10(-7) M, respectively. Trace metal requirements for cobalt, chromium, and copper were also demonstrated, but minimum concentrations could not be determined because other reagents contributed a high background of these metals. Under certain conditions an apparent response to zinc was observed, although other experiments suggest the zinc salt contained another metal that was required for growth. Poly-beta-hydroxybutyrate biosynthesis was shown to be initiated by a magnesium or sulfate deficiency as well as by a nitrogen or phosphate deficiency.
确定了真养产碱菌无限制自养生长的定量营养需求。使光密度增加2时，Mg2+、SO42-、PO43-、Fe3+和Na2+的最低饱和浓度分别为10(-4)M、8×10(-5)M、5×10(-4)至6×10(-4)M、10(-5)M以及10(-7)至2×10(-7)M。还证明了对钴、铬和铜的痕量金属需求，但由于其他试剂导致这些金属的背景值较高，所以无法确定最低浓度。在某些条件下，观察到对锌有明显反应，不过其他实验表明锌盐中含有生长所需的另一种金属。聚-β-羟基丁酸酯的生物合成显示可由镁或硫酸盐缺乏以及氮或磷酸盐缺乏引发。
