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利用酶联免疫吸附测定法测定某些按蚊的人体血液指数。

Determination of the human blood index of some anopheline mosquitos by using ELISA.

作者信息

Adugna N, Petros B

机构信息

Ethiopian Health and Nutrition Research Institute, Addis Ababa, Ethiopia.

出版信息

Ethiop Med J. 1996 Jan;34(1):1-10.

PMID:8674495
Abstract

To determine the human blood index, 1253 anopheline mosquitos collected from Arbaminch, Awassa, Metahara and Ziway were tested by the blood meal ELISA, based on anti-human IgG. Higher positivity for human blood were observed in anopheline mosquitos collected from mixed dwellings (range, 26-92%) while those from animal shelters had the lowest positivity (range, 9-48%). The human blood index for A. arabiensis, a principal malaria vector in most parts of the country, from mixed dwelling collections was 88% while it was 43% for those collected from animal shelters. For A. pharoensis, it was 84% and 9%, and for A. coustani, it was 26% and 15% for the respective sites of collection. The human blood index of A. marshalli was 40%; for A. demeilloni, it was 36%; and for A. christyi, 91% for collections from mixed dwellings, while 35% for A. tenebrosus was determined for those collected from animal shelters. Out of the 15 A. funestus and the 11 A. longipalpis tested, 13 and 8 were positive for human blood, respectively. As the feeding and resting preference of the mosquitos varied, malaria control measures must be based on integrated measures in order to reduce man-mosquito contact.

摘要

为确定人类血液指数,对从阿尔巴明奇、阿瓦萨、梅塔哈拉和齐瓦采集的1253只按蚊进行了基于抗人IgG的血餐ELISA检测。从混合住所采集的按蚊中观察到人类血液阳性率较高(范围为26%-92%),而从动物庇护所采集的按蚊阳性率最低(范围为9%-48%)。该国大部分地区主要疟疾传播媒介阿拉伯按蚊从混合住所采集样本的人类血液指数为88%,而从动物庇护所采集样本的该指数为43%。对于法老按蚊,相应采集地点的该指数分别为84%和9%;对于库斯塔尼按蚊,分别为26%和15%。马歇尔按蚊的人类血液指数为40%;德梅洛尼按蚊为36%;克里斯蒂按蚊从混合住所采集样本的该指数为91%,而从动物庇护所采集的黑暗按蚊该指数为35%。在检测的15只险恶按蚊和11只长须按蚊中,分别有13只和8只人类血液检测呈阳性。由于蚊子的摄食和栖息偏好各不相同,疟疾控制措施必须基于综合措施,以减少人与蚊子的接触。

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