Feuille F, Ebersole J L, Kesavalu L, Stepfen M J, Holt S C
Department of Periodontics, University of Texas Health Science Center at San Antonio, USA.
Infect Immun. 1996 Jun;64(6):2094-100. doi: 10.1128/iai.64.6.2094-2100.1996.
These studies determined the characteristics of tissue destruction in a murine abscess model elicited by mixed infection with the periodontopathogens Fusobacterium nucleatum and Porphyromonas gingivalis. The interbacterial effects of this synergism, the kinetics of the relationship of the bacterial interaction, and the characteristics of the bacteria required for the tissue destruction were studied. Infection of mice with P. gingivalis and F. nucleatum strains elicited lesions of various sizes as a function of infective dose. Primary infection with F. nucleatum plus P. gingivalis at various ratios (i.e., <1:1) resulted in a significantly greater lesion size (P < 0.001) compared with that resulting from primary infection with P. gingivalis alone. At F. nucleatum/P. gingivalis ratios of > or = 1:1, spreading lesion formation and progression were significantly (P < 0.001) decreased, suggesting that bacterial interaction (i.e., coaggregation) may have inhibited the spread of the P. gingivalis infection to a site distant from the initial injection. Infection with F. nucleatum and P. gingivalis simultaneously (at different sites) or F. nucleatum administered within 4 h prior to or 1 h following P. gingivalis infection significantly enhanced the ability of P. gingivalis to form large phlegmonous lesions. Chemical inhibition of the P. gingivalis trypsin-like protease activity or the use of a trypsin-negative P. gingivalis strain abrogated tissue destruction either alone or in combination with F. nucleatum. Therefore, it was possible to examine aspects of virulence of these pathogens in a murine lesion model by either altering bacterial ratios, manipulating the time of infection, or targeting vital bacterial virulence factors.
这些研究确定了在由牙周病原体具核梭杆菌和牙龈卟啉单胞菌混合感染引发的小鼠脓肿模型中组织破坏的特征。研究了这种协同作用的细菌间效应、细菌相互作用关系的动力学以及组织破坏所需细菌的特征。用牙龈卟啉单胞菌和具核梭杆菌菌株感染小鼠会引发大小各异的病变，病变大小是感染剂量的函数。以不同比例（即<1:1）用具核梭杆菌加牙龈卟啉单胞菌进行初次感染，与单独用牙龈卟啉单胞菌进行初次感染相比，病变大小显著更大（P<0.001）。当具核梭杆菌/牙龈卟啉单胞菌的比例≥1:1时，扩展性病变的形成和进展显著（P<0.001）减少，这表明细菌相互作用（即共聚）可能抑制了牙龈卟啉单胞菌感染扩散到远离初始注射部位的地方。同时（在不同部位）用具核梭杆菌和牙龈卟啉单胞菌感染，或在牙龈卟啉单胞菌感染前4小时内或感染后1小时内给予具核梭杆菌，均显著增强了牙龈卟啉单胞菌形成大的蜂窝织炎性病变的能力。化学抑制牙龈卟啉单胞菌的胰蛋白酶样蛋白酶活性或使用胰蛋白酶阴性的牙龈卟啉单胞菌菌株，单独或与具核梭杆菌联合使用时均可消除组织破坏。因此，通过改变细菌比例、控制感染时间或靶向关键的细菌毒力因子，有可能在小鼠病变模型中研究这些病原体的毒力方面。
