抗李斯特菌溶血素的中和单克隆抗体：参与孔形成的表位图谱分析
Neutralizing monoclonal antibodies against listeriolysin: mapping of epitopes involved in pore formation.
作者信息
Darji A, Niebuhr K, Hense M, Wehland J, Chakraborty T, Weiss S
机构信息
Department of Cell Biology and Immunology, National Research Centre for Biotechnology, Braunschweig, Germany.
出版信息
Infect Immun. 1996 Jun;64(6):2356-8. doi: 10.1128/iai.64.6.2356-2358.1996.
Abstract
Six different mouse monoclonal antibodies (MAbs) and a specific rabbit polygonal antibody were raised against listeriolysin. Four of the MAbs also recognized seeligeriolysin, and five cross-reacted with ivanolysin. The hemolytic activity could be neutralized by the polygonal antibody as well as by five of the MAbs. None of the neutralizing antibodies interfered with the binding of listeriolysin to the cellular membrane. The epitopes recognized by the MAbs were localized by using overlapping synthetic peptides between positions 59 and 279, a region hitherto not implicated in mediating hemolytic activity.
摘要
针对李斯特菌溶血素制备了六种不同的小鼠单克隆抗体（MAb）和一种特异性兔多克隆抗体。其中四种单克隆抗体也识别海氏李斯特菌溶血素，五种与伊万诺夫溶血素发生交叉反应。多克隆抗体以及五种单克隆抗体均可中和溶血活性。所有中和抗体均不干扰李斯特菌溶血素与细胞膜的结合。利用59至279位之间的重叠合成肽确定了单克隆抗体识别的表位，该区域迄今未被认为与介导溶血活性有关。
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本文引用的文献
1
Hyperexpression of listeriolysin in the nonpathogenic species Listeria innocua and high yield purification.李斯特菌溶血素在无害李斯特菌非致病物种中的过表达及高产纯化。
J Biotechnol. 1995 Dec 15;43(3):205-12. doi: 10.1016/0168-1656(95)00138-7.
2
Listeriolysin is a potent inducer of the phosphatidylinositol response and lipid mediator generation in human endothelial cells.李斯特菌溶血素是人类内皮细胞中磷脂酰肌醇反应和脂质介质生成的有效诱导剂。
Infect Immun. 1996 Feb;64(2):674-6. doi: 10.1128/iai.64.2.674-676.1996.
3
Detection of a prfA-independent promoter responsible for listeriolysin gene expression in mutant Listeria monocytogenes strains lacking the PrfA regulator.在缺乏PrfA调节因子的单核细胞增生李斯特菌突变株中检测负责李斯特菌溶血素基因表达的不依赖PrfA的启动子。
Infect Immun. 1993 Jul;61(7):3073-5. doi: 10.1128/iai.61.7.3073-3075.1993.
4
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Infect Immun. 1993 Jun;61(6):2545-52. doi: 10.1128/iai.61.6.2545-2552.1993.
5
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Mol Biol Cell. 1994 Apr;5(4):455-64. doi: 10.1091/mbc.5.4.455.
6
A role in cell-binding for the C-terminus of pneumolysin, the thiol-activated toxin of Streptococcus pneumoniae.肺炎链球菌的硫醇激活毒素肺炎溶血素C末端在细胞结合中的作用。
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7
Inhibition of human polymorphonuclear leukocyte respiratory burst, bactericidal activity, and migration by pneumolysin.肺炎溶血素对人多形核白细胞呼吸爆发、杀菌活性及迁移的抑制作用。
Infect Immun. 1983 Sep;41(3):1212-6. doi: 10.1128/iai.41.3.1212-1216.1983.
8
Suppression of chemotatic activity of human neutrophils by streptolysin O.链球菌溶血素O对人中性粒细胞趋化活性的抑制作用。
J Infect Dis. 1972 Apr;125(4):353-9. doi: 10.1093/infdis/125.4.353.
9
Mechanism of membrane damage by streptolysin-O.链球菌溶血素O造成膜损伤的机制。
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10
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