肺炎溶血素对人多形核白细胞呼吸爆发、杀菌活性及迁移的抑制作用。
Inhibition of human polymorphonuclear leukocyte respiratory burst, bactericidal activity, and migration by pneumolysin.
作者信息
Paton J C, Ferrante A
出版信息
Infect Immun. 1983 Sep;41(3):1212-6. doi: 10.1128/iai.41.3.1212-1216.1983.
Abstract
The in vitro effects of pneumolysin, a sulfhydryl-activated toxin produced by Streptococcus pneumoniae, on various functions of human polymorphonuclear leukocytes (PMNLs) was investigated. Treatment of PMNLs with highly purified toxin significantly inhibited respiratory burst (in response to stimulation), ability to kill opsonized pneumococci, chemotaxis, and random migration. These inhibitions were observed at very low toxin doses (less than or equal to 1 hemolytic unit (2 ng) per 10(6) PMNLs), which had no effect on PMNL viability. These results suggest that pneumolysin could function in pathogenicity by interfering with the ability of PMNLs to migrate toward and kill pneumococci.
摘要
研究了肺炎链球菌产生的巯基激活毒素——肺炎溶血素对人多形核白细胞（PMNLs）各种功能的体外作用。用高度纯化的毒素处理PMNLs可显著抑制呼吸爆发（对刺激的反应）、杀死调理过的肺炎球菌的能力、趋化性和随机迁移。在非常低的毒素剂量下（每10⁶个PMNLs小于或等于1个溶血单位（2纳克））就观察到了这些抑制作用，而这些剂量对PMNLs的活力没有影响。这些结果表明，肺炎溶血素可能通过干扰PMNLs向肺炎球菌迁移并杀死它们的能力而在致病性中发挥作用。
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