Tang P, Rosenshine I, Cossart P, Finlay B B
Biotechnology Laboratory, Department of Biochemistry and Molecular Biology, University of British Columbia, Vancouver, Canada.
Infect Immun. 1996 Jun;64(6):2359-61. doi: 10.1128/iai.64.6.2359-2361.1996.
Infection with Listeria monocytogenes induces the activation of mitogen-activated protein (MAP) kinase in several tissue culture cell lines (P.Tang, I. Rosenshine, and B. B. Finlay, Mol. Biol. Cell 5:455-464, 1994). After various mutants were examined, the bacterial factor responsible for MAP kinase activation was identified as listeriolysin O (LLO). Growth supernatant containing LLO or purified LLO alone can induce MAP kinase tyrosine phosphorylation in HeLa cells. Single-amino-acid mutations in LLO that do not affect its membrane binding capacity but reduce its cytolytic activity also reduced its ability to induce MAP kinase activity in HeLa cells. Streptolysin O, another sulfhydryl-activated hemolysin, and the detergent saponin are also able to activate MAP kinase in target cells. Thus, the increased MAP kinase activity observed in L. monocytogenes-infected cells is most likely a result of the permeabilization of the host cell membrane by LLO and may not be linked with invasion.
单核细胞增生李斯特菌感染可诱导多种组织培养细胞系中的丝裂原活化蛋白（MAP）激酶激活（P.唐、I.罗森欣和B.B.芬利，《分子生物学细胞》5:455 - 464，1994）。在检测了各种突变体后，确定负责MAP激酶激活的细菌因子为李斯特菌溶血素O（LLO）。含有LLO的生长上清液或单独的纯化LLO均可诱导HeLa细胞中MAP激酶酪氨酸磷酸化。LLO中不影响其膜结合能力但降低其细胞溶解活性的单氨基酸突变，也降低了其在HeLa细胞中诱导MAP激酶活性的能力。另一种巯基活化溶血素链球菌溶血素O和去污剂皂角苷也能够激活靶细胞中的MAP激酶。因此，在单核细胞增生李斯特菌感染细胞中观察到的MAP激酶活性增加很可能是LLO使宿主细胞膜通透性增加的结果，可能与侵袭无关。
