双胍类药物对卡氏棘阿米巴包囊和滋养体的致死作用。
The lethal effects of biguanides on cysts and trophozoites of Acanthamoeba castellanii.
作者信息
Khunkitti W, Lloyd D, Furr J R, Russell A D
机构信息
Welsh School of Pharmacy, University of Wales College of Cardiff, UK.
出版信息
J Appl Bacteriol. 1996 Jul;81(1):73-7. doi: 10.1111/j.1365-2672.1996.tb03284.x.
The effects of a range of biocides on trophozoite and encysted forms of Acanthamoeba castellanii were investigated. Viable acanthamoebae were enumerated by a plaque assay technique. The cyst form of Acanthamoeba castellanii was more resistant to all biocides tested than the trophozoite form. Of the biocides tested, chlorhexidine diacetate (CHA) and polyhexamethylene biguanide (PHMB) were the most effective. Their lethal effects were time- and concentration-dependent. CHA was very effective when formulated in 0.1% EDTA combined with Tris buffer pH 7.8 whereas PHMB activity was reduced by 0.1% EDTA. Three per cent dimethylsulphoxide (DMSO) enhanced the activity of CHA but not of PHMB.
研究了一系列杀生物剂对卡氏棘阿米巴滋养体和包囊形式的影响。通过蚀斑试验技术对存活的棘阿米巴进行计数。卡氏棘阿米巴的包囊形式比滋养体形式对所有测试的杀生物剂更具抗性。在所测试的杀生物剂中，双醋酸氯己定（CHA）和聚六亚甲基双胍（PHMB）最为有效。它们的致死作用具有时间和浓度依赖性。当在0.1%乙二胺四乙酸（EDTA）与pH 7.8的Tris缓冲液中配制时，CHA非常有效，而0.1%的EDTA会降低PHMB的活性。3%的二甲基亚砜（DMSO）增强了CHA的活性，但没有增强PHMB的活性。
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