Khunkitti W, Avery S V, Lloyd D, Furr J R, Russell A D
Welsh School of Pharmacy, University of Wales Cardiff, UK.
J Antimicrob Chemother. 1997 Aug;40(2):227-33. doi: 10.1093/jac/40.2.227.
The lethal effects of various biocides, in particular two agents (chlorhexidine diacetate and polyhexamethylene biguanide) on cysts and trophozoites of Acanthamoeba castellanii have been measured by both plaque assay and flow cytometry (FCM). Minimum amoebicidal concentrations against trophozoites were generally the same by the two methods whereas minimum cysticidal concentrations were occasionally higher by FCM. Concentrations of biguanides required to produce an equal reduction in viability were also higher by FCM than by plaque assay.
通过噬斑测定法和流式细胞术（FCM）测定了各种杀生物剂，特别是两种药剂（双醋酸氯己定和聚六亚甲基双胍）对卡氏棘阿米巴囊肿和滋养体的致死作用。两种方法测得的针对滋养体的最低杀阿米巴浓度通常相同，而通过FCM测得的最低杀囊肿浓度偶尔会更高。与噬斑测定法相比，FCM测定产生同等活力降低所需的双胍类药物浓度也更高。
