Higaki S, Nakamura M, Morohashi M, Hasegawa Y, Yamagishi T
Department of Dermatology, Faculty of Medicine, Toyama Medical and Pharmaceutical University, Japan.
J Dermatol. 1996 May;23(5):310-4. doi: 10.1111/j.1346-8138.1996.tb04021.x.
Anti-lipase activity of Kampo formulations, Coptidis Rhizoma (CR), and its alkaloids against Propionibacterium acnes were examined in vitro. The amounts of propionic and butyric acids in the medium were measured as growth and lipase activity of P. acnes, respectively. In tributyrin-PYG medium with each concentration of Kampo formulation, CR, or the alkaloids added, the production of propionic acid was suppressed remarkably more than that of butyric acid. The suppression of production of these acids by CR was higher than that of the alkaloids. Furthermore, no lipase-negative colonies were found on the medium to which Kampo formulations were added. From these observations, we concluded that not only Kampo formulations and CR, but also their alkaloids, showed suppression of growth of P. acnes, which reduced anti-lipase activity. Furthermore, it was suggested that Kampo formulations and Kampo crude drugs with anti-lipase activity to P. acnes should be synergistic when their ingredients are combined.
研究了汉方制剂、黄连（CR）及其生物碱对痤疮丙酸杆菌的抗脂肪酶活性。分别测定培养基中丙酸和丁酸的含量，作为痤疮丙酸杆菌的生长和脂肪酶活性指标。在添加了不同浓度汉方制剂、CR或生物碱的三丁酸甘油酯-PYG培养基中，丙酸的产生受到的抑制明显大于丁酸。CR对这些酸产生的抑制作用高于生物碱。此外，在添加汉方制剂的培养基上未发现脂肪酶阴性菌落。从这些观察结果来看，我们得出结论，不仅汉方制剂和CR，而且它们的生物碱也显示出对痤疮丙酸杆菌生长的抑制作用，这降低了抗脂肪酶活性。此外，还表明对痤疮丙酸杆菌具有抗脂肪酶活性的汉方制剂和汉方生药在其成分组合时应具有协同作用。
