Higaki S, Hasegawa Y, Morohashi M, Sakamoto K, Yamagishi T
Department of Dermatology, Faculty of Medicine, Toyama Medical and Pharmaceutical University.
Nihon Hifuka Gakkai Zasshi. 1990 Jul;100(8):883-6.
Gas chromatography was applied for determination of the amounts of propionic and butyric acids in the medium produced by Propionibacterium acnes. The organism was incubated in Peptone-Yeast extract-Glucose medium containing 0.017 mEq/ml of tributyrin and various amounts of Coptidis Rhizoma at 37 degrees C for 24 to 96 hr. The amount of butyric acid produced by the lipase was reduced parallel with that of propionic acid resulted from the growth of P. acnes in the medium. These facts were also confirmed by counting the cell numbers of P. acnes. Furthermore, any lipase-negative colonies were not observed on the Kishishita's Lipase Medium. These findings indicate that Coptidis Rhizoma inhibits growth of P. acens and anti-lipase activity of the drug against the organism is resulted from the fact.
采用气相色谱法测定痤疮丙酸杆菌在培养基中产生的丙酸和丁酸含量。将该菌株在含有0.017 mEq/ml三丁酸甘油酯和不同量黄连的蛋白胨 - 酵母提取物 - 葡萄糖培养基中于37℃培养24至96小时。脂肪酶产生的丁酸量与痤疮丙酸杆菌在培养基中生长产生的丙酸量平行减少。通过计算痤疮丙酸杆菌的细胞数量也证实了这些事实。此外，在岸下氏脂肪酶培养基上未观察到任何脂肪酶阴性菌落。这些发现表明黄连抑制痤疮丙酸杆菌的生长，并且该药物对该菌的抗脂肪酶活性源于这一事实。
