Lind M, Overgaard S, Søballe K, Nguyen T, Ongpipattanakul B, Bünger C
Department of Orthopaedics, University Hospital of Arhus, Denmark.
J Orthop Res. 1996 May;14(3):343-50. doi: 10.1002/jor.1100140303.
Growth of bone into cementless prosthetic components is compromised after revision of failed joint prostheses and by osteoporosis, gaps, and micromotion. We studied the effects of recombinant human transforming growth factor-beta 1 adsorbed on ceramic coated implants on the improvement of mechanical fixation and bone growth on the implant. Unloaded cylindrical grit-blasted titanium alloy implants were inserted bilaterally into both the medial and lateral femoral condyles of 10 skeletally mature mongrel dogs. The implants measured 10 mm in length and 6 mm in diameter and were initially surrounded by a 2 mm gap. One implant had an uncoated titanium surface and three implants were coated with tricalcium phosphate and 0, 0.3, or 3.0 micrograms of recombinant human transforming growth factor-beta 1. The dogs were killed at 6 weeks. Mechanical testing showed a 3-fold increase in fixation for the 0.3 microgram dose of recombinant human transforming growth factor-beta 1 and a 2-fold increase for the 3.0 micrograms dose. Histological analysis of bone growth on the implant demonstrated that maximal stimulation occurred with the 0.3 microgram dose, but bone volume in the gap was maximally stimulated by the 3.0 micrograms dose and increased 2-fold over control values. The majority of tricalcium phosphate was resorbed after the 6-week observation period. This study suggests that recombinant human transforming growth factor-beta 1 adsorbed onto implants coated with tricalcium phosphate ceramic can enhance mechanical fixation and bone growth on the implant. The use of transforming growth factor-beta 1 on ceramic coated prosthetic components may help to improve the functional outcome of cementless total joint replacements.
在翻修失败的关节假体后，以及在骨质疏松、存在间隙和微动的情况下，骨长入非骨水泥假体部件的过程会受到影响。我们研究了吸附在陶瓷涂层植入物上的重组人转化生长因子-β1对改善植入物的机械固定和骨生长的作用。将未加载的圆柱形喷砂钛合金植入物双侧插入10只骨骼成熟的杂种狗的股骨内外侧髁。植入物长度为10毫米，直径为6毫米，最初周围有2毫米的间隙。一个植入物具有未涂层的钛表面，三个植入物涂有磷酸三钙，并分别吸附了0、0.3或3.0微克的重组人转化生长因子-β1。在6周时处死这些狗。机械测试表明，0.3微克剂量的重组人转化生长因子-β1使固定力增加了3倍，3.0微克剂量使固定力增加了2倍。对植入物上骨生长的组织学分析表明，0.3微克剂量产生了最大刺激，但间隙中的骨体积在3.0微克剂量时受到最大刺激，比对照值增加了2倍。在6周观察期后，大部分磷酸三钙被吸收。这项研究表明，吸附在磷酸三钙陶瓷涂层植入物上的重组人转化生长因子-β1可以增强植入物的机械固定和骨生长。在陶瓷涂层假体部件上使用转化生长因子-β1可能有助于改善非骨水泥全关节置换的功能结果。
