Lind M, Overgaard S, Nguyen T, Ongpipattanakul B, Bünger C, Søballe K
Department of Orthopedics, University Hospital of Aarhus, Denmark.
Acta Orthop Scand. 1996 Dec;67(6):611-6. doi: 10.3109/17453679608997766.
Unloaded cylindrical grit-blasted titanium (Ti-6A-4V) implants (6 x 10 mm) coated with hydroxyapatite ceramic were inserted into the proximal part of the humerus of 20 skeletally mature Labrador dogs. The implants were initially surrounded by a 2 mm gap. In 10 dogs, HA-coated implants without growth factor were inserted in one humerus and implants with 0.3 microgram rhTGF-beta 1 adsorbed onto the HA coating were inserted in the contralateral humerus. In another group of 10 dogs, a dose of 3.0 micrograms rhTGF-beta 1 was tested in a similar design. All dogs were killed at 6 weeks after treatment. Results were evaluated by histomorphometry and mechanical push-out testing. Bone ongrowth was increased by one third, using the 0.3 mg rhTGF-beta 1 stimulation. Bone volume in the gap and mechanical testing showed no statistically significant differences between control and rhTGF-beta 1 stimulated implants. RhTGF-beta 1 only moderately enhanced bone ongrowth to hydroxyapatite-coated implants.
将涂覆有羟基磷灰石陶瓷的无负载圆柱形喷砂钛（Ti-6A-4V）植入物（6×10毫米）插入20只骨骼成熟的拉布拉多犬肱骨近端。植入物最初被2毫米的间隙包围。在10只狗中，将未添加生长因子的HA涂层植入物插入一侧肱骨，将吸附有0.3微克rhTGF-β1的HA涂层植入物插入对侧肱骨。在另一组10只狗中，以类似设计测试了3.0微克rhTGF-β1的剂量。所有狗在治疗后6周处死。通过组织形态计量学和机械推出试验评估结果。使用0.3毫克rhTGF-β1刺激，骨长入增加了三分之一。间隙中的骨体积和机械测试显示，对照植入物和rhTGF-β1刺激的植入物之间无统计学显著差异。RhTGF-β1仅适度增强了骨向羟基磷灰石涂层植入物的长入。
