Tortorella C, Polignano A, Piazzolla G, Serrone M, Jirillo E, Antonaci S
Department of Internal Medicine, Immunology and Infectious Diseases, University of Bari Medical Faculty, Policlinico, Italy.
Microbios. 1996;85(344):189-98.
The impairment of superoxide anion (O2-) generation by aged polymorphonuclear cells (PMN) stimulated with formyl-methionyl-leucine-phenylalanine (FMLP) has been reported. In this work the effect of lipopolysaccharide (LPS), granulocyte-macrophage colony stimulating factor (GM-CSF) and pentoxifylline (POF) pretreatment on FMLP-triggered neutrophil oxidative metabolism in a group of healthy elderly individuals, was investigated. Results provide evidence that LPS and/or GM-CSF priming was able to enhance O2- production in old PMN, even if values were still lower than those observed in similarly-treated young cells. Moreover, even if the lag period was unaffected by inflammatory mediator treatment, the priming gave rise to a significant increase of maximum O2- release rate. On the contrary, POF pretreatment led to a significant decrease of oxidative responsiveness in either unprimed or primed PMN suspensions. These findings suggest the occurrence of different mechanisms in the imbalance of FMLP-activated neutrophil oxidative responsiveness during senescence. This may be of paramount significance for explaining the augmented frequency of severe infectious diseases with advancing age.
据报道，衰老的多形核白细胞（PMN）受甲酰甲硫氨酰亮氨酰苯丙氨酸（FMLP）刺激后超氧阴离子（O2-）生成受损。在这项研究中，研究了脂多糖（LPS）、粒细胞巨噬细胞集落刺激因子（GM-CSF）和己酮可可碱（POF）预处理对一组健康老年人中FMLP触发的中性粒细胞氧化代谢的影响。结果表明，LPS和/或GM-CSF预处理能够增强老年PMN中O2-的产生，即使其值仍低于在同样处理的年轻细胞中观察到的值。此外，即使延迟期不受炎症介质处理的影响，但预处理导致最大O2-释放速率显著增加。相反，POF预处理导致未预处理或预处理的PMN悬液中的氧化反应性显著降低。这些发现表明，衰老过程中FMLP激活的中性粒细胞氧化反应性失衡存在不同机制。这对于解释随着年龄增长严重传染病频率增加可能具有至关重要的意义。
