来自母鸡蛋黄的一种蛋白酶抑制剂是一种卵类粘蛋白类似物。

A proteinase inhibitor from egg yolk of hen is an ovoinhibitor analog.

作者信息

Sugimoto Y, Kusakabe T, Nagaoka S, Nirasawa T, Tatsuguchi K, Fujii M, Aoki T, Koga K

机构信息

Department of Food and Nutrition, Seinan-Jogakuin Junior College, Kitakyushu, Japan.

出版信息

Biochim Biophys Acta. 1996 Jun 7;1295(1):96-102. doi: 10.1016/0167-4838(96)00034-9.

DOI:10.1016/0167-4838(96)00034-9

PMID:8679679

Abstract

A proteinase inhibitor, tentatively termed vitelloinhibitor, was purified from yolk of hen's ovarian follicles. It resembled egg-white ovoinhibitor not only in inhibitory spectrum (active for bovine trypsin and bovine chymotrypsin) but also in thermal stability, pH stability, antiserum reactivity and amino-acid composition. However, vitelloinhibitor had different molecular weight from that of ovoinhibitor. An alpha 2-proteinase inhibitor preparation, isolated from laying hen's serum in the present study, was found to exhibit two bands, and the larger one of the latter corresponded to vitelloinhibitor in molecular weight. The partial N-terminal amino-acid sequence of vitelloinhibitor was the same as those of the two components of serum inhibitor and all three agreed with that of ovoinhibitor. Vitelloinhibitor is likely to be an ovoinhibitor analog derived from a serum precursor, which might be the larger component of alpha 2-proteinase inhibitor.

摘要

一种蛋白酶抑制剂，暂称为卵黄抑制剂，是从母鸡卵巢卵泡的卵黄中纯化得到的。它不仅在抑制谱（对牛胰蛋白酶和牛胰凝乳蛋白酶有活性）方面与蛋清中的卵类粘蛋白抑制剂相似，而且在热稳定性、pH稳定性、抗血清反应性和氨基酸组成方面也相似。然而，卵黄抑制剂的分子量与卵类粘蛋白抑制剂不同。在本研究中从产蛋母鸡血清中分离得到的一种α2蛋白酶抑制剂制剂显示出两条带，其中较大的一条在分子量上与卵黄抑制剂相对应。卵黄抑制剂的部分N端氨基酸序列与血清抑制剂的两个组分相同，并且这三者都与卵类粘蛋白抑制剂一致。卵黄抑制剂可能是一种源自血清前体的卵类粘蛋白抑制剂类似物，该血清前体可能是α2蛋白酶抑制剂的较大组分。