Herremans A H, Hijzen T H, Welborn P F, Olivier B, Slangen J L
CNS-Pharmacology, DA Weesp, The Netherlands.
Brain Res. 1996 Mar 4;711(1-2):102-11. doi: 10.1016/0006-8993(95)01404-7.
Rats trained on a Delayed Matching To Position (DMTP) task displayed mediating behavior during delays to solve the task. Infusion of the cholinergic antagonist scopolamine into the medial Prefrontal Cortex area (mPFC), dose dependently impaired performance independent of delay. These results indicate that scopolamine does not specifically affect working memory. Infusion of the cholinesterase inhibitor physostigmine, muscarinic subtype receptor antagonists, the dopamine (D1) antagonist SCH23390, and of the GABA-A receptor antagonist bicuculline, did not affect performance in the DMTP task. In a post-hoc analysis scopolamine was found to impair discriminability in a delay-dependent manner only in animals that used mediating behavior in the majority of the trials. Furthermore, a time sampling method indicated that scopolamine infusions into the mPFC disrupted mediating behavior during the task. Results suggest that cholinergic systems in the mPFC play a role in directing attention to task relevant behavior.
在延迟位置匹配（DMTP）任务中接受训练的大鼠在延迟期间表现出中介行为以解决任务。向内侧前额叶皮质区域（mPFC）注入胆碱能拮抗剂东莨菪碱，剂量依赖性地损害了表现，且与延迟无关。这些结果表明东莨菪碱不会特异性地影响工作记忆。注入胆碱酯酶抑制剂毒扁豆碱、毒蕈碱亚型受体拮抗剂、多巴胺（D1）拮抗剂SCH23390和GABA - A受体拮抗剂荷包牡丹碱，均未影响DMTP任务中的表现。在事后分析中发现，仅在大多数试验中使用中介行为的动物中，东莨菪碱以延迟依赖性方式损害辨别能力。此外，一种时间采样方法表明向mPFC注入东莨菪碱会在任务期间破坏中介行为。结果表明，mPFC中的胆碱能系统在将注意力导向与任务相关的行为方面发挥作用。
