黄花蒿法呢基二磷酸合酶编码cDNA的克隆与分析
Cloning and analysis of a cDNA encoding farnesyl diphosphate synthase from Artemisia annua.
作者信息
Matsushita Y, Kang W, Charlwood B V
机构信息
Division of Life Sciences, King's College London, UK.
出版信息
Gene. 1996 Jun 26;172(2):207-9. doi: 10.1016/0378-1119(96)00054-6.
A cDNA encoding farnesyl diphosphate (FPP) synthase (FPPS) has been cloned from a cDNA library of Artemisia annua. The sequence analysis showed that the cDNA encoded a protein of 343 amino acid (aa) residues with a calculated molecular weight of 39420 kDa. The deduced aa sequence of the cDNA was highly similar to FPPS from other plants, yeast and mammals, and contained the two conserved domains found in polyprenyl synthases including FPPS, geranylgeranyl diphosphate synthases and hexaprenyl diphosphate synthases. The expression of the cDNA in Escherichia coli showed enzyme activity for FPPS in vitro.
已从黄花蒿的cDNA文库中克隆出一个编码法尼基二磷酸（FPP）合酶（FPPS）的cDNA。序列分析表明，该cDNA编码一个由343个氨基酸残基组成的蛋白质，计算分子量为39420 kDa。该cDNA推导的氨基酸序列与其他植物、酵母和哺乳动物的FPPS高度相似，并包含在聚异戊二烯合酶（包括FPPS、香叶基香叶基二磷酸合酶和六异戊二烯基二磷酸合酶）中发现的两个保守结构域。该cDNA在大肠杆菌中的表达显示出体外FPPS的酶活性。
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