荚膜红细菌nifF基因的克隆、特性分析及调控
Cloning, characterization, and regulation of nifF from Rhodobacter capsulatus.
作者信息
Gennaro G, Hübner P, Sandmeier U, Yakunin A F, Hallenbeck P C
机构信息
Département de Microbiologie et Immunologie, Université de Montréal, Canada.
出版信息
J Bacteriol. 1996 Jul;178(13):3949-52. doi: 10.1128/jb.178.13.3949-3952.1996.
Abstract
The Rhodobacter capsulatus nifF gene and upstream sequence were cloned by using a probe based on the N-terminal sequence of NifF. nifF was found to not be contained in the previously described nif regions I, II, and III. Comparison of the deduced amino acid sequence showed that it is highly similar to NifF from Azotobacter vinelandii and NifF from Klebsiella pneumoniae. Analysis of translational fusions demonstrated that the regulation of transcription was the same as previously reported at the protein level. Insertional mutagen esis showed that NifF contributes significantly to nitrogenase activity under normal nitrogen-fixing conditions and that it is absolutely required for nitrogen fixation under iron limitation.
摘要
利用基于固氮铁氧还蛋白N端序列的探针克隆了荚膜红细菌的nifF基因及其上游序列。发现nifF并不包含在先前描述的nif区域I、II和III中。推导的氨基酸序列比较表明，它与棕色固氮菌的固氮铁氧还蛋白和肺炎克雷伯菌的固氮铁氧还蛋白高度相似。翻译融合分析表明，转录调控与先前在蛋白质水平上报道的相同。插入诱变表明，在正常固氮条件下，固氮铁氧还蛋白对固氮酶活性有显著贡献，并且在铁限制条件下固氮绝对需要它。
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