肺炎克雷伯菌nifF基因的碱基序列及其蛋白质产物预测氨基酸序列与其他黄素氧还蛋白的同源性。
The base sequence of the nifF gene of Klebsiella pneumoniae and homology of the predicted amino acid sequence of its protein product to other flavodoxins.
作者信息
Drummond M H
出版信息
Biochem J. 1985 Dec 15;232(3):891-6. doi: 10.1042/bj2320891.
Abstract
The nucleotide sequence of a 629 base-pair segment of DNA spanning the nifF gene of Klebsiella pneumoniae is presented. The structural gene comprises 531 base-pairs (175 codons, excluding the translational initiator and terminator) encoding an acidic polypeptide of 18950 Da. The nifF product thus belongs to the long-chain class of flavodoxins. It shows some sequence homology to the short-chain flavodoxins from Desulfovibrio vulgaris, Clostridium MP and Megasphaera elsdenii, and much stronger homology to long-chain flavodoxins from Azotobacter vinelandii and Anacystis nidulans. The long chain flavodoxins thus seem to constitute a well-conserved sub-group. The homology with the A. vinelandii flavodoxin is particularly strong, which may reflect their common function in nitrogen fixation.
摘要
本文给出了肺炎克雷伯菌nifF基因所在的一段629个碱基对的DNA序列。该结构基因由531个碱基对组成（175个密码子，不包括翻译起始密码子和终止密码子），编码一个18950 Da的酸性多肽。因此，nifF产物属于长链类黄素氧还蛋白。它与来自普通脱硫弧菌、梭菌MP和埃氏巨球形菌的短链黄素氧还蛋白有一些序列同源性，与来自棕色固氮菌和集胞藻的长链黄素氧还蛋白有更强的同源性。因此，长链黄素氧还蛋白似乎构成了一个保守性良好的亚组。与棕色固氮菌黄素氧还蛋白的同源性尤为强烈，这可能反映了它们在固氮中的共同功能。
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